Fitc anti mouse tcr β clone h57 597

Manufactured by BD

Sourced in United States

The FITC anti-mouse TCR β clone H57-597 is a fluorescence-labeled monoclonal antibody that binds to the T cell receptor beta chain (TCR β) expressed on the surface of mouse T cells. This product can be used for the identification and enumeration of mouse T cells in flow cytometry applications.

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Spelling variants of this product

H57-597 (9 citations) Mouse anti (2 citations)

2 protocols using fitc anti mouse tcr β clone h57 597

Murine Lymphocyte Characterization by Flow Cytometry

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Following preparation of single‐cell suspensions, the cells were incubated with anti‐mouse CD16/32 clone 93 (BioLegend) for blocking of Fc‐receptors to avoid nonspecific binding. Lymphocytes were stained with fluorescent antibodies and tetramers for an hour. The following antibodies/tetramers were used for staining: FITC rat anti‐mouse CD1d clone 1B1 (BD Biosciences, Franklin Lakes, NJ), FITC rat IgG2b k isotype control (BD Biosciences), FITC anti‐mouse TCR β clone H57‐597 (BD Biosciences), PE PBS‐57 loaded tetramer and unloaded tetramer (kindly provided by the NIH Tetramer Core, Emory, GA), APC anti‐mouse CD122 clone TM‐b1 (eBioscience, San Diego, CA), APC CD3e anti‐mouse clone 145‐2C11 (BD Biosciences), PE‐Cy7 anti‐mouse CD25 clone PC61 (BioLegend), PE‐Cy7 anti‐mouse CD4 clone RM4‐5 (BioLegend), APC anti‐mouse CD8a clone 53‐6.7 (BioLegend), APC anti‐mouse CD69 clone H1.2F3 (BD Biosciences), PE‐Cy5 anti‐mouse CD5 clone 53‐7.3 (BioLegend), PerCP‐Cy5.5 anti‐mouse CD44 clone IM7 (BioLegend), PE‐Cy7 anti‐mouse CD24 clone M1/69 (BioLegend). Flow cytometric analysis was performed using a BD FACS Verse and a BD LSR II flow cytometer. The results were analyzed in FlowJo version 9.5.3 (TreeStar, Ashland, OR).

Schrumpf E., Jiang X., Zeissig S., Pollheimer M.J., Anmarkrud J.A., Tan C., Exley M.A., Karlsen T.H., Blumberg R.S, & Melum E. (2017). The role of natural killer T cells in a mouse model with spontaneous bile duct inflammation. Physiological Reports, 5(4), e13117.

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Lymphocyte Immune Phenotyping Protocol

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Following preparation of single-cell suspensions, the cells were incubated with anti-mouse CD16/32 clone 93 (BioLegend, San Diego, CA, USA) for blocking of Fc-receptors to avoid non-specific binding. Lymphocytes were stained with FITC anti-mouse TCRβ, clone H57-597 (BD Biosciences, Franklin Lakes, NJ, USA) for an hour. Flow cytometric analysis was performed using a BD FACS Verse flow cytometer (BD Biosciences). The results were analysed in FlowJo version 9.5.3 (TreeStar, Ashland, OR, USA). Detailed anti-body information is stated in Supplementary Table 2.

Schrumpf E., Kummen M., Valestrand L., Greiner T.U., Holm K., Arulampalam V., Reims H.M., Baines J., Bäckhed F., Karlsen T.H., Blumberg R.S., Hov J.R, & Melum E. (2016). THE GUT MICROBIOTA CONTRIBUTES TO A MOUSE MODEL OF SPONTANEOUS BILE DUCT INFLAMMATION. Journal of hepatology, 66(2), 382-389.

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