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Agilent 2100 bioanalyser rna 6000 nano kit

Manufactured by Agilent Technologies
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The Agilent 2100 Bioanalyzer RNA 6000 Nano kit is a lab equipment product used for the analysis and quantification of RNA samples. It provides a sensitive and automated method for assessing the quality and integrity of RNA samples prior to further experimental procedures.

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Lab products found in correlation

Nebnext ultra 2 directional rna library prep kit, by Illumina (1 mentions) Rnaeasy kit, by Qiagen (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Rt for pcr kit, by Takara Bio (1 mentions)

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Agilent 2100 Bioanalyser (554 citations) 2100 Bioanalyser (440 citations) Agilent Bioanalyser (81 citations) RNA 6000 Nano (61 citations) Agilent RNA 6000 Nano (23 citations) Agilent 2100 RNA 6000 Nano kit (14 citations) Bioanalyser Nano Kit (3 citations) Agilent 2100 Bioanalyser (Agilent RNA 6000 Nano Kit (2 citations) Bioanalyser RNA Nano 6000 kit (2 citations) Nano 6000 kit (2 citations)

2 protocols using agilent 2100 bioanalyser rna 6000 nano kit

1

RNA Sequencing Library Preparation

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RNA samples were QCed using the Agilent 2100 Bioanalyser RNA 6000 Nano kit and libraries were made using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina kits. Subsequent libraries were QCed using the Agilent 2100 Bioanalyser High Sensitivity Assay and the Qubit fluorometer. Pooled libraries were run on a Paired End 75 + 8 bp dual index High Output NextSeq 550 run to generate 50 million reads per sample.
Jobbins A.M., Haberman N., Artigas N., Amourda C., Paterson H.A., Yu S., Blackford S.J., Montoya A., Dore M., Wang Y.F., Sardini A., Cebola I., Zuber J., Rashid S.T., Lenhard B, & Vernia S. (2022). Dysregulated RNA polyadenylation contributes to metabolic impairment in non-alcoholic fatty liver disease. Nucleic Acids Research, 50(6), 3379-3393.
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2

RNA Extraction and cDNA Synthesis

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RNA was extracted from RNAlater-preserved normal skin and CMCT samples using either TRIzol (Life Technologies) or the RNAeasy kit (QIAGEN). RNA integrity was evaluated by microfluidic electrophoresis (Agilent 2100 Bioanalyser RNA 6000 Nano Kit). cDNA was synthesised using the RT-for-PCR kit (Clontech).
Arendt M.L., Melin M., Tonomura N., Koltookian M., Courtay-Cahen C., Flindall N., Bass J., Boerkamp K., Megquir K., Youell L., Murphy S., McCarthy C., London C., Rutteman G.R., Starkey M, & Lindblad-Toh K. (2015). Genome-Wide Association Study of Golden Retrievers Identifies Germ-Line Risk Factors Predisposing to Mast Cell Tumours. PLoS Genetics, 11(11), e1005647.
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