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Phospho t389 s6 kinase

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The Phospho-T389 S6 kinase is a lab equipment product that detects the phosphorylation of the Thr389 residue on the S6 kinase protein. This phosphorylation event is critical for the activation of S6 kinase, a key regulator of cell growth and protein synthesis. The product can be used to measure the activity and regulation of this important signaling protein.

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Lab products found in correlation

β actin, by Cell Signaling Technology (3 mentions) Tristetraprolin, by Cell Signaling Technology (2 mentions) α tubulin, by Merck Group (2 mentions) Phospho erk thr202 tyr204, by Cell Signaling Technology (2 mentions) C myc, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Brf1 2, by Cell Signaling Technology (2 mentions) Psat1, by Novus Biologicals (1 mentions) Phospho ampkα t172, by Cell Signaling Technology (1 mentions) Phospho ulk1 s757, by Cell Signaling Technology (1 mentions) S6 ribosomal protein, by Cell Signaling Technology (1 mentions) Phgdh, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Ampkα, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Phospho-S6 (110 citations) Phospho-p70 S6 Kinase T389 (7 citations) Phospho-p70 S6 Kinase T389 (108D (2 citations)

4 protocols using phospho t389 s6 kinase

1

Western Blot Analysis of Metabolic Enzymes

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Cells were lysed in buffer containing 50 mM Tris pH 7.4, 1% Nonidet P-40, 0.25% sodium deoxycholate, 1 mM EDTA, 150 mM NaCl, 1 mM dithiothreitol, 1 mM sodium orthovanadate, 20 mM sodium fluoride, 2 μg ml−1 aprotinin, 2 μg ml−1 leupeptin and 0.7 μg ml−1 pepstatin. Western blot analysis was performed using standard protocols, and the following commercial antibodies were used as probes: ASNS (Proteintech 14681-1-AP, 1:1,000), phospho-T389 S6 kinase (Cell Signaling 9234, 1:500), S6 kinase (Cell Signaling 2708, 1:1,000), phospho-S235/235 S6 ribosomal protein (Cell Signaling 4858, 1:3,000), S6 ribosomal protein (Cell Signaling 2217, 1:1,000), LC3A/B (Cell Signaling 4108, 1:1,000), PHGDH (Cell Signaling 13428, 1:1,000), PSAT1 (Abnova H00029968-A01, 1:500), PSPH (Sigma HPA020376, 1:500), SHMT1 (Abcam ab55736, 1:1,000), SHMT2 (Cell Signaling 12762, 1:1,000), PRPS2 (Abnova H00005634-A01, 1:1,000), 4E-BP1 (Cell Signaling 9452, 1:1,000) and α-tubulin (Sigma T6074, 1:10,000). Immunoblot images have been cropped for presentation. Full-size images are presented in Supplementary Fig. 6.
Krall A.S., Xu S., Graeber T.G., Braas D, & Christofk H.R. (2016). Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor. Nature Communications, 7, 11457.
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2

Western Blot Analysis of Cellular Signaling

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Cells were lysed in buffer containing 50 mM Tris pH 7.4, 1% Nonidet P-40, 0.25% sodium deoxycholate, 1 mM EDTA, 150 mM NaCl, 1 mM dithiothreitol, 1 mM sodium orthovanadate, 20 mM sodium fluoride, 10 mM beta-glycerophosphate, 10 mM sodium pyrophosphate, 2 ug ml−1 aprotinin, 2 ug ml−1 leupeptin and 0.7 ug ml−1 pepstatin. Western blot analysis was performed using standard protocols, and the following commercial antibodies were used as probes: ASNS (Proteintech 14681-1-AP, 1:1000), ATF4 (Cell Signaling 11815, 1:500), phospho-T389 S6 kinase (Cell Signaling 9234, 1:500), S6 kinase (Cell Signaling 2708, 1:1000), phospho-S235/235 S6 ribosomal protein (Cell Signaling 4858, 1:3000), S6 ribosomal protein (Cell Signaling 2217, 1:1000), phospho-S1859 CAD (Cell Signaling 70307, 1:500), CAD (Cell Signaling 11933, 1:1000), 4E-BP1 (Cell Signaling 9644, 1:500), phospho-S757 ULK1 (Cell Signaling 14202, 1:1000), ULK1 (Cell Signaling 8054, 1:1000), phospho-T172 AMPKα (Cell Signaling 2535, 1:500), AMPKα (Cell Signaling 2532, 1:1000), PHGDH (Cell Signaling 13428, 1:500), β-Actin (Cell Signaling 4970, 1:1000), PSAT1 (Novus 89-004-606, 1:500), and a-tubulin (Sigma T6074, 1:10,000).
Krall A.S., Mullen P.J., Surjono F., Momcilovic M., Schmid E.W., Halbrook C.J., Thambundit A., Mittelman S.D., Lyssiotis C.A., Shackelford D.B., Knott S.R, & Christofk H.R. (2021). Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth. Cell metabolism, 33(5), 1013-1026.e6.
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3

Protein Extraction and Western Blotting

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Cells were lysed in RIPA buffer supplemented with 2μg/mL aprotinin, 2μg/mL leupeptin, 0.7μg/mL pepstatin, 20mM sodium fluoride, 1mM sodium orthovanadate, 1mM dithiothreitol, 10mM beta-glycerophosphate, and 10mM sodium pyrophosphate. Following protein quantification using Bradford reagent, western blots were performed using standard procedures. The following commercially available antibodies were used for immunoblotting: tristetraprolin (Cell Signaling Technology 71632, 1:500), BRF1/2 (Cell Signaling Technology 2119, 1:1000), ERK (Cell Signaling Technology 9102, 1:1000), phospho-Thr202/Tyr204 ERK (Cell Signaling Technology 4370, 1:1000), S6 kinase (Cell Signaling Technology 2708, 1:1000), phospho-T389 S6 kinase (Cell Signaling Technology 9234, 1:500), c-Myc (Cell Signaling Technology 18583, 1:1000), GAPDH (Cell Signaling Technology 5174, 1:1000), β-actin (Cell Signaling Technology 3700, 1:1000) and α-tubulin (Sigma T6074, 1:10,000).
Cicchetto A.C., Jacobson E.C., Sunshine H., Wilde B.R., Krall A.S., Jarrett K.E., Sedgeman L., Turner M., Plath K., Luisa Iruela-Arispe M., de Aguiar Vallim T.Q, & Christofk H.R. (2023). ZFP36-mediated mRNA decay regulates metabolism. Cell reports, 42(5), 112411.
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4

Protein Extraction and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer supplemented with 2μg/mL aprotinin, 2μg/mL leupeptin, 0.7μg/mL pepstatin, 20mM sodium fluoride, 1mM sodium orthovanadate, 1mM dithiothreitol, 10mM beta-glycerophosphate, and 10mM sodium pyrophosphate. Following protein quantification using Bradford reagent, western blots were performed using standard procedures. The following commercially available antibodies were used for immunoblotting: tristetraprolin (Cell Signaling Technology 71632, 1:500), BRF1/2 (Cell Signaling Technology 2119, 1:1000), ERK (Cell Signaling Technology 9102, 1:1000), phospho-Thr202/Tyr204 ERK (Cell Signaling Technology 4370, 1:1000), S6 kinase (Cell Signaling Technology 2708, 1:1000), phospho-T389 S6 kinase (Cell Signaling Technology 9234, 1:500), c-Myc (Cell Signaling Technology 18583, 1:1000), GAPDH (Cell Signaling Technology 5174, 1:1000), β-actin (Cell Signaling Technology 3700, 1:1000) and α-tubulin (Sigma T6074, 1:10,000).
Cicchetto A.C., Jacobson E.C., Sunshine H., Wilde B.R., Krall A.S., Jarrett K.E., Sedgeman L., Turner M., Plath K., Luisa Iruela-Arispe M., de Aguiar Vallim T.Q, & Christofk H.R. (2023). ZFP36-mediated mRNA decay regulates metabolism. Cell reports, 42(5), 112411.
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