Mixed-meal tolerance tests (MMTTs) were performed 14 days prior to and 13 days post-surgery. Mice were fasted for 6 h and tail vein blood was collected for basal blood glucose and insulin measurements. Subsequently, mice were gavaged with 200 μL of Osmolite HiCal liquid diet enriched with 20% dextrose. Tail vein blood was collected after 15, 30, 60, and 120 min to assess glucose and circulating insulin levels during the MMTT. Blood glucose was quantified in plasma using mouse glucose assay kit (CrystalChem, #81692). Levels of circulating insulin were assayed in plasma using mouse-specific ELISA kits (CrystalChem, #90080).
Mouse specific elisa kits
Manufactured by Crystal Chem
Sourced in United States
Mouse-specific ELISA kits are laboratory tools designed to quantify target analytes in mouse samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure specific proteins or other biomolecules of interest. The core function of these kits is to provide a standardized and reliable method for researchers to analyze mouse-derived samples.
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Lab products found in correlation
2 protocols using mouse specific elisa kits
1
Glucose and Insulin Dynamics in Mice
2
Quantifying GLP-1 and Insulin Levels
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Total GLP-1 and insulin levels in plasma samples were determined using mouse-specific ELISA kits (Crystal Chem, IL, USA) as per the manufacturer’s guidelines. For the GLP-1 ELISA, 10 µL of plasma diluted 1:5 in buffer solution was used for the assay. For the insulin ELISA, 5 µL of plasma was used per sample. Results are presented as absolute values for GLP-1 and change (delta) in insulin compared to 0-min timepoint for insulin.
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