The enrolled children were recalled at least once during their childhood phase. All the outcome assessments were performed at each visit. The height (±0.1 cm) and weight (±0.1 kg) of the offspring were measured twice using a stadiometer and electronic scale with a light cloth and no shoes. The BMI was calculated as weight (kg)/[height (m)]2. Blood samples of the offspring were collected after overnight fasting and stored at −80°C until the tests. Fasting blood glucose (FBG) levels were analyzed using the hexokinase method (Cobas c702 instrument; Roche Diagnostics, Germany). Fasting insulin was measured in the serum using an electrochemiluminescence immunoassay (Cobas e601 instrument; Roche Diagnostics, Germany). Total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and LDL-C levels were measured in serum using a homogeneous assay (Cobas c702 instrument; Roche Diagnostics, Germany). HOMA-IR was calculated as [insulin (mIU/L) FBG (mmol/L)]/22.5. HOMA of beta-cell function (HOMA-β) was calculated using the formula [20*insulin (mIU/mL)]/[FBG (mmol/L) - 3.5].
Cobas c702 instrument
Manufactured by Roche
Sourced in Germany
The Cobas c702 instrument is a fully automated clinical chemistry analyzer designed for high-volume laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests, including enzymatic, immunochemical, and ion-selective electrode measurements. The Cobas c702 instrument is designed to provide accurate and reliable results, with a focus on efficiency and productivity for high-throughput laboratory environments.
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3 protocols using cobas c702 instrument
1
Childhood Growth and Metabolic Outcomes
2
Quantifying COVID-19 Immune Markers
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Levels of anti-SARS-CoV2-spike IgG antibodies were measured by an enzyme-linked immunosorbent assay (ELISA) using the Elecsys immunoassay (Roche Diagnostics, Mannheim, Germany) following the manufacturer’s specifications. A positive result was indicated by a value > 0.4 U/mL and the upper measurement limit was set to 2500 U/mL. C-reactive protein (CRP) was measured in serum by an article-enhanced immunological turbidity test, where the aggregates are determined turbidimetrically (Cobas instrument, Roche Diagnostics, Mannheim, Germany). Interleukin-6 (IL-6) was analyzed in plasma samples using the Immunological ECLIA test (ElektroChemiLuminescence ImmunoAssay) on the Cobas instrument (Roche Diagnostics, Mannheim, Germany). Activated Partial Thromboplastin Time (aPTT; reference value: 26–36 s) was measured photometrically (Pathromtin SL, Siemens Healthcare); D-dimers (reference value: <500 µg/L) were analyzed in citrate plasma by a particle-enhanced immunoturbidimetric assay (Innovance D-Dimer Kit, Siemens Healthcare). Anti-β2glycoprotein IgG antibodies and anticardiolipin antibodies were measured by a quantitative ELISA (Euroimmun, Lübeck, Germany). Glucose concentrations were determined in serum using the GLUC3 assay on a Cobas c702 instrument (Roche Diagnostics GmbH, Mannheim, Germany).
3
Biomarker Measurement in Serum
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C-reactive protein (CRP; reference range 0-0.5 mg/dL) was measured by an article-enhanced immunological turbidity test, where the aggregates are determined turbidimetrically (Cobas instrument, Roche Diagnostics, Mannheim, Germany). Interleukin-6 (IL-6; reference range <15 pg/mL) was measured from serum samples using the Immunological ECLIA test (ElektroChemiLuminescence ImmunoAssay) on Cobas instrument (Roche Diagnostics, Mannheim, Germany). Serum creatinine was analyzed by the Jaffe method. Glucose levels were measured in serum using the GLUC3 assay on a Cobas c702 instrument (Roche Diagnostics GmbH, Mannheim, Germany).
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