The 5-8F, HONE1, SPC-A-1 and A549 cells were purchased from Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China) and all cultured in RPMI-1640 medium (Corning, NY, USA) supplemented with 10% fetal bovine serum (FBS, GIBCO, South America, NY, USA). All these cells were maintained with 5% CO2 atmosphere at 37°C.
Hone1
Manufactured by Shanghai Cell Bank
Sourced in China
The HONE1 is a high-performance cell culture incubator designed for use in cell biology laboratories. It maintains precise temperature, humidity, and gas concentration control to create an optimal environment for cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
C666 1, by Shanghai Cell Bank (2 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Spc a 1, by Shanghai Cell Bank (1 mentions)
Gammacell 40, by Nordion (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hek293t, by Shanghai Cell Bank (1 mentions)
5 protocols using hone1
1
Cell Culture Protocol for Cancer Research
2
Establishment of Radioresistant NPC Cell Lines
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NPC cell lines of CNE2 and HONE1 were purchased from Shanghai Cell Bank in 2016. CNE2R, a radioresistant human NPC cell line, was previously constructed and maintained at our laboratory [30 (link)]. Briefly, CNE2 cells were irradiated with fractionated doses (2, 2, 4, 4, 4, 4, 6, 6, 6, 6, 8, 8 Gy) of γ-ray irradiation (137-Cs, Gammacell-40, MDS Nordion, Canada) at a dose rate of 0.73 Gy/min. Between two exposures, cells were cultured for nearly 7 days to recovery. After the last irradiation, the survived cells became more radioresistant than their parent cells, which was named CNE2-R cells. The cells were cultured with RPMI-1640 medium (Gibco, Hangzhou, China) supplied with 10% fetal bovine serum (Gibco Invitrogen, Grand Island, NY, United States), 100 U/mL penicillin, and 100 mg/mL streptomycin, and maintained at 37 °C in a humidified atmosphere of 5% CO2 and 95% O2.
3
Nasopharyngeal Carcinoma Tissue Collection
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The study was approved by the Institutional Ethical Review Boards of Shanghai Ninth People’s Hospital and complied with the guidelines of the Declaration of Helsinki. A total of 56 NPC tissues and adjacent normal tissues with informed consent were collected by biopsy between July 2016 and June 2018. None of the patients had received any chemotherapy or radiotherapy before biopsy. NPC cell lines, including HONE-1, C666-1, CNE-1, CNE-2, and SUNE-1, and human immortalized nasopharyngeal epithelial cell line (NP69) were purchased from the Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). Cells were incubated at 37°C and supplemented with 5% CO2 in a humidified incubator.
4
Establishment of Radioresistant NPC Cell Lines
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Fresh NPC tissues were obtained from the First Affiliated Hospital of Guangxi Medical University and approved by the appropriate ethics committee. Informed consent was obtained from all NPC patients before specimen collection. The two NPC cell lines (C666-1 and HONE1) used in this study were obtained from Shanghai Cell Bank, cultured in RPMI-1640 medium (Gibco, Hangzhou, China) supplemented with 10% fetal bovine serum (FBS; Gibco, New York, USA), and incubated at 37 °C in an atmosphere with 5% CO2. Mycoplasma was administered once per month to eliminate contamination.
The two relatively radiation-resistant sublines C666-1R and HONE1R were established in our laboratory by irradiating C666-1 and HONE1 cells with a fractionated dose of X-rays (137Cs, Gammacell-40, Varian, Canada) of 4 Gy for 15 times (60 Gy in total) at a rate of 400 cGy/min. Cells were irradiated once per week. After fractionated irradiation at 60 Gy, the surviving cells, named C666-1R and HONE1R cells, were more radioresistant than the corresponding parent cells, which are C666-1 and HONE1.
The two relatively radiation-resistant sublines C666-1R and HONE1R were established in our laboratory by irradiating C666-1 and HONE1 cells with a fractionated dose of X-rays (137Cs, Gammacell-40, Varian, Canada) of 4 Gy for 15 times (60 Gy in total) at a rate of 400 cGy/min. Cells were irradiated once per week. After fractionated irradiation at 60 Gy, the surviving cells, named C666-1R and HONE1R cells, were more radioresistant than the corresponding parent cells, which are C666-1 and HONE1.
5
Cell Culture Protocol for Various Cell Lines
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Human nasal epithelial cell (HNEpC), human embryonic kidney cell (HEK‐293T), and the NPC cells (HONE1, CNE1, CNE2, and 5‐8F) were all purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (China). All cells, except that HEK‐293T cells were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco), were maintained in RPMI 1640 medium (Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% antibiotics (penicillin/streptomycin; HyClone). Cell culture was conducted at 37°C in 5% CO2, along with the change of medium every three days.
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