Sc 395739

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-395739 is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a core function in scientific research and analysis. The detailed specifications and intended use of this product are not available.

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Lab products found in correlation

Control crispr cas9 plasmid, by Santa Cruz Biotechnology (1 mentions) Sc 418922, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sc 395739

Targeted N-SMase2 Knockdown in Mouse Brain

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To disrupt N-SMase2 we downregulated Smpd3 gene in the brain via intra-cortical injection of CRISPR-Cas9/Homology directed repair (HDR) plasmid. First, we standardized injection protocol by visualizing the distribution of fluorescence signal in coronally sectioned brains following bilateral intra-cortical injection of 1ul Red CellTracker™ CM-Dil (ThermoFisher) into the cerebral cortex of P7 mice (1 mm from the Bregma and 1 mm from the sagittal suture).
The CRISPR mix solution containing 0.3 μg N-SMase2 CRISPR-Cas9-KO plasmid (sc-425544, Santa Cruz Biotechnology) or Control CRISPR-Cas9 plasmid (sc-41892, Santa Cruz Biotechnology) as a negative control, was diluted 1:1 in transfection reagent (sc-395739, Santa Cruz Biotechnology) containing N-SMase2 HDR Plasmid (sc-425544-HDR, Santa Cruz Biotechnology) just before intra-cortical injection. 1 μl CRISPR-Cas9-HDR or CRISPR-Cas9/KO- HDR plasmid per left and right cortex was injected over 1 min using dispenser (PB600–1, Hamilton Company Inc.) at the coordinates defined above.

Lecuyer M., Pathipati P., Faustino J, & Vexler Z.S. (2021). Neonatal stroke enhances interaction of microglia-derived extracellular vesicles with microglial cells. Neurobiology of disease, 157, 105431.

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CRISPR-mediated ENDOG Modulation in MDA-MB-453

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Clustered regularly interspaced short palindromic repeats (CRISPR) transfection of ENDOG in MDA-MB-453 was performed using a kit from Santa Cruz (sc-395739, Santacruz Biotechnology, USA). Briefly, in six-well culture plates, 10⁶ cells were plated and exposed to the ENDOG plasmid (sc-403263, Santacruz Biotechnology, USA) or negative control-CRISPR plasmid (sc-418922, Santacruz Biotechnology, USA) solution for 8 h at 37 ℃ in a CO₂ incubator. Then, media was changed to Dulbecco’s MEM with 10% fetal bovine serum and incubated for another 18 h. The ENDOG expression was determined using RT-PCR.

Kim H., Choi J.H., Moon C.M., Kang J.L., Woo M, & Kim M. (2022). Shrimp miR-965 transfers tumoricidal mitochondria. Biological Procedures Online, 24, 16.

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CRISPR and Overexpression of Bcl-2/Bcl-xL in Lung Cancer

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CRISPR or wild type transfection of Bcl-2 and Bcl-xL in human lung cancer cells was performed using a kit from Santa Cruz (sc-395739, Santacruz, USA). Briefly, in six-well culture plates, 10⁶ cells were plated and exposed to the Bcl-2 wild type plasmid (8768, RRID: Addgene_8768, Addgene, USA), Bcl-xL wild type plasmid (113458, RRID: Addgene_113458, Addgene, USA), Bcl-2 CRISPR plasmid (sc-400025-KO-2, Santacruz, USA), Bcl-xL CRISPR plasmid (sc-400170, Santacruz, USA) or control plasmid (sc-418922, Santacruz, USA) solution for 8 h at 37 °C in a CO₂ incubator. Then, media was changed to Dulbecco modified eagle medium (DMEM) with 10% bovine serum and incubated for another 12 h.

, & Kim M. (2024). Mitochondria of T Lymphocytes Promote Anti-Pulmonary Tumor Immune Response. World Journal of Oncology, 15(3), 472-481.

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