Log In Sign up
The largest database of trusted experimental protocols

Paav ef1a fdio cre

Manufactured by Addgene
Visit Supplier
Sourced in United States

The PAAV-EF1a-fDIO-Cre is a lentiviral vector for Cre recombinase expression. It contains the EF1a promoter and a 'floxed' (flanked by loxP) inverted Cre recombinase gene.

Automatically generated - may contain errors

Lab products found in correlation

Paav hsyn dio egfp, by Addgene (3 mentions) Paav hsyn dio mcherry, by Addgene (2 mentions) Paav syn flex gcamp6f wpre sv40, by Addgene (2 mentions) Aav ef1a dio gcamp6f p2a nls tdtomato, by Addgene (2 mentions) Paav hsyn1 sio stgtacr2 fusionred, by Addgene (2 mentions) Paav ef1a double floxed hchr2 h134r eyfp wpre hghpa, by Addgene (1 mentions) Paav ef1a flpo, by Addgene (1 mentions)

3 protocols using paav ef1a fdio cre

1

Stereotactic AAV Virus Injections in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
The AAV-viruses used in this study include: AAV2-Y444F-CAG-DIO-mWGA-mCherry (VIROVEK, Cat# 117-UCSF86), pAAV-EF1a-double-floxed-hChR2 (H134R)-EYFP-WPRE-HGHpA (Addgene, Cat# 20298-AAV9), pAAV-hSyn-DIO-EGFP (Addgene, Cat# 50457-AAVrg), AAV pEF1a-DIO-FLPo-WPRE-hGHpA (Addgene, Cat# 87306-AAVrg), pAAV-EF1a-Flpo (Addgene, Cat# 55637-AAVrg), pAAV-EF1a-fDIO-Cre (Addgene, Cat# 121675-AAV9), pAAV-Ef1a-fDIO-GCaMP6f (Addgene, Cat# 128315-AAV1), pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (Addgene, Cat# 154868-AAV8), pAAV-Ef1a-fDIO mCherry (Addgene, Cat# 55641-AAV1), and pAAV-CMV-fDIO-TeNT-EYFP (obtained from Dr. Wei Xu at UT Southwestern Medical Center). AAV virus was injected using a Nanoject III® with 1nl/s speed stereotactically in 5–7 weeks old male mice under isoflurane anesthesia. The injection coordinates used for the DVC were: AP: −7.5 mm from bregma; mediolateral (ML): ± 0.5 mm; dorsoventral (DV): −4.9 mm. The injection coordinates used for the PVN were: AP: −0.92 mm from bregma; ML: ± 0.18 mm; DV: −4.75 mm. Viral-mediated gene expression was allowed for 3–4 weeks before experimental manipulation. Injection sites were confirmed post hoc by cutting brain sections and inspecting them under a stereoscope or microscope in all animals reported in this study.
Wang L., Savani R., Bernabucci M., Lu Y., Singh I., Xu W., El Ouaamari A., Wheeler M.B., Grill H.J., Rossi M.A, & Pang Z.P. (2024). State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis. Research Square.
+ Open protocol
+ Expand
2

Diverse Viral Tools for Neuroscience

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The rAAV5-Ef1a-DIO-hChR2-eYFP, AAV9-CAG-Flex-GFP, and AAV8-hSyn-DIO-KORD-IRES-mCitrine were produced by the University of North Carolina vector core facility (Chapel Hill, North Carolina, USA). The pAAV-Syn-Flex-GCaMP6f-WPRE-SV40 (Catalog number 100833), AAV-Ef1a-DIO-GCaMP6f-P2A-nls-tdTomato (retrograde AAV; Catalog number 51083), pAAV-EF1a-fDIO-Cre (retrograde AAV; Catalog number 121675-AAVrg), pAAV_hSyn1-SIO-stGtACR2-FusionRed (Catalog number 105677-AAV1), pAAV-hSyn-DIO-EGFP (retrograde AAV; Catalog number 50457-AAVrg) and pAAV-hSyn-DIO-mCherry (retrograde AAV; Catalog number 50459-AAVrg) were produced by Addgene (Watertown, MA, USA). The CAV-Flex-FlpO was produced by Montpellier vector platform (Plateforme de Vectorologie de Montpellier (PVM), Biocampus Montpellier, Montpellier, France). The AAVDJ-CAG-fDIO-oG-WPRE-SV40pA was produced by the Viral Vector Core Facility at Salk Institute. The AAV2/8-Ef1a-fDIO-TVA-mCherry was produced by Z. Josh Huang’s lab at CSHL. The Rbv-CVS-N2c-dG-GFP (the optimized rabies virus)36 (link) was produced by HHMI Janelia Research Campus. The AAV8-CaMKIIa-FDO-GCaMP6f (Flp-off) virus was produced by K. Deisseroth’s lab at Stanford University. All viral vectors were aliquoted and stored at –80 °C until use.
Zhang X., Guan W., Yang T., Furlan A., Xiao X., Yu K., An X., Galbavy W., Ramakrishnan C., Deisseroth K., Ritola K., Hantman A., He M., Huang Z.J, & Li B. (2021). Genetically identified amygdala-striatal circuits for valence-specific behaviors. Nature neuroscience, 24(11), 1586-1600.
+ Open protocol
+ Expand
3

Diverse Viral Tools for Neuroscience

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The rAAV5-Ef1a-DIO-hChR2-eYFP, AAV9-CAG-Flex-GFP, and AAV8-hSyn-DIO-KORD-IRES-mCitrine were produced by the University of North Carolina vector core facility (Chapel Hill, North Carolina, USA). The pAAV-Syn-Flex-GCaMP6f-WPRE-SV40 (Catalog number 100833), AAV-Ef1a-DIO-GCaMP6f-P2A-nls-tdTomato (retrograde AAV; Catalog number 51083), pAAV-EF1a-fDIO-Cre (retrograde AAV; Catalog number 121675-AAVrg), pAAV_hSyn1-SIO-stGtACR2-FusionRed (Catalog number 105677-AAV1), pAAV-hSyn-DIO-EGFP (retrograde AAV; Catalog number 50457-AAVrg) and pAAV-hSyn-DIO-mCherry (retrograde AAV; Catalog number 50459-AAVrg) were produced by Addgene (Watertown, MA, USA). The CAV-Flex-FlpO was produced by Montpellier vector platform (Plateforme de Vectorologie de Montpellier (PVM), Biocampus Montpellier, Montpellier, France). The AAVDJ-CAG-fDIO-oG-WPRE-SV40pA was produced by the Viral Vector Core Facility at Salk Institute. The AAV2/8-Ef1a-fDIO-TVA-mCherry was produced by Z. Josh Huang’s lab at CSHL. The Rbv-CVS-N2c-dG-GFP (the optimized rabies virus)36 (link) was produced by HHMI Janelia Research Campus. The AAV8-CaMKIIa-FDO-GCaMP6f (Flp-off) virus was produced by K. Deisseroth’s lab at Stanford University. All viral vectors were aliquoted and stored at –80 °C until use.
Zhang X., Guan W., Yang T., Furlan A., Xiao X., Yu K., An X., Galbavy W., Ramakrishnan C., Deisseroth K., Ritola K., Hantman A., He M., Huang Z.J, & Li B. (2021). Genetically identified amygdala-striatal circuits for valence-specific behaviors. Nature neuroscience, 24(11), 1586-1600.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!