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Sterile tissue culture plates

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Sterile tissue culture plates are a type of laboratory equipment used for the in vitro cultivation of cells, tissues, or microorganisms. These plates provide a controlled and contamination-free environment for the growth and maintenance of these biological samples.

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Lab products found in correlation

Anti tgf β, by BioXCell (2 mentions) Anti cd28, by BioLegend (2 mentions) Anti cd3ε 145 2c11, by BioLegend (2 mentions) Jes6 1a12, by BioXCell (2 mentions) Recombinant human tgf β, by Thermo Fisher Scientific (1 mentions) Recombinant human il 2, by Thermo Fisher Scientific (1 mentions) Rhtgf β, by Thermo Fisher Scientific (1 mentions) Rhil 2, by Thermo Fisher Scientific (1 mentions) 70 μm cell strainer, by BD (1 mentions)

Spelling variants of this product

Tissue culture plates (66 citations) White polystyrene flat bottom sterile 384-well tissue culture treated plates (3 citations) 6-well sterile tissue culture plates (2 citations)

4 protocols using sterile tissue culture plates

1

CD4+ T-cell Activation and Differentiation

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24 well sterile tissue culture plates (Corning, NY, USA) were coated with 4 µg/ml anti-CD3ε and 4 µg/ml anti-CD28 in 0.5 ml/well PBS at 37 °C for 2 h.
Purified CD4+ T-cells were washed using complete RPMI medium twice to eliminate the MACS buffer and then resuspended in complete RPMI media supplemented with 100 IU/ml recombinant human IL-2 (Peprotech; Rocky Hill, NJ, USA) and 5 ng/ml recombinant human TGF-β (Peprotech). 3 × 105 cells were added to the wells at a volume of 1 ml/well. Cells were cultured at 37 °C 5% CO2 for 3 days.
Akkaya B., Holstein A.H., Isaac C., Maz M.P., Glass D.D., Shevach E.M, & Akkaya M. (2016). Ex-vivo iTreg differentiation revisited: Convenient alternatives to existing strategies. Journal of immunological methods, 441, 67-71.
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2

T Cell Differentiation and Expansion Protocols

Cited 2 times
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For iTreg and Tactivated cell differentiation, naive T cells were isolated and 24 well sterile tissue culture plates (Corning, Corning, NY) were coated anti-CD3ε (145–2C11, Biolegend) and anti-CD28 (37.51, Biolegend) as described 36 (link). Naive CD4+ T-cells were resuspended in complete RPMI media supplemented with 100 IU/ml recombinant human IL-2 for both iTreg and Tactivated, with additional 5 ng/ml recombinant human TGF-β for iTreg and 10 μg/ml anti-TGF-β (1D11.16.8, BioXcell, West Lebanon, NH) for Tactivated cultures. 3 × 105 cells were added to the wells at a volume of 1 ml/well. Cells were cultured at 37°C 5% CO2 for 3 days. Prior to experiments, live Tactivated and iTreg were FACS sorted based on their Foxp3-GFP expression status.
IL-2/anti–IL-2 mAb (JES6-1-A12, Bioxcell) complexes were prepared and injected as in 37 (link) to expand antigen-specific tTregs in vivo. Antigen-specific Tregs were isolated from spleens of the TCR transgenic animals by FACS sorting based on Foxp3 reporter expression. Alternatively, antigen-specific tTregs were expanded in vitro. For in vitro proliferation, Tregs were cultured for three days in the presence of plate bound anti-CD3ε (145–2C11; 2 ug/mL), anti-CD28 (37.51; 2 ug/mL) and IL-2 (100 U/mL). On day 3, cells were split 1:2 and cultured with only IL-2. Tregs were harvested on day 5 and FACS sorted for Foxp3 reporter.
Akkaya B., Oya Y., Akkaya M., Souz J.A., Holstein A.H., Kamenyeva O., Kabat J., Matsumura R., Dorward D.W., Glass D.D, & Shevach E.M. (2019). T regulatory cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells. Nature immunology, 20(2), 218-231.
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3

Differentiation of Naïve CD4+ T Cells

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6–8 weeks male Tipe2 KO and WT mice (five per experimental group) were sacrificed and spleens were collected into sterile complete RPMI. Single cell suspensions were obtained by mashing the spleen and passing cells through 70 μm cell strainer (BD, Franklin Lakes NJ, USA). Naïve CD4+ T cells were obtained according to the protocol of Miltenyi. 24 well sterile tissue culture plates (Corning, NY, USA) were coated with 4 μg/mL anti-CD3ε and 4 μg/mL anti-CD28 in 0.5 mL/well PBS at 37 °C for 2 h. Purified naïve CD4+ T cells which was isolated from Tipe2-/- or WT spleen were washed and resuspended in complete RPMI media supplemented with 100 IU/mL rhIL-2 (Peprotech, Cat#AF-200-02-10, Rocky Hill, NJ, USA) and 5 ng/mL rhTGF-β (Peprotech, Cat#100-21C-2). 3 × 105 cells were added to the wells at a volume of 1 mL/well. Cells were cultured at 37 °C 5% CO2 for 3 days and harvested for flow cytometry.
Li Y., Zhang N., Ma C., Xu W., Jin G., Zheng Y., Zhang L., Liu B., Gao C, & Liu S. (2021). The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation. Cell Death & Disease, 12(11), 1001.
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4

T Cell Differentiation and Expansion Protocols

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
For iTreg and Tactivated cell differentiation, naive T cells were isolated and 24 well sterile tissue culture plates (Corning, Corning, NY) were coated anti-CD3ε (145–2C11, Biolegend) and anti-CD28 (37.51, Biolegend) as described 36 (link). Naive CD4+ T-cells were resuspended in complete RPMI media supplemented with 100 IU/ml recombinant human IL-2 for both iTreg and Tactivated, with additional 5 ng/ml recombinant human TGF-β for iTreg and 10 μg/ml anti-TGF-β (1D11.16.8, BioXcell, West Lebanon, NH) for Tactivated cultures. 3 × 105 cells were added to the wells at a volume of 1 ml/well. Cells were cultured at 37°C 5% CO2 for 3 days. Prior to experiments, live Tactivated and iTreg were FACS sorted based on their Foxp3-GFP expression status.
IL-2/anti–IL-2 mAb (JES6-1-A12, Bioxcell) complexes were prepared and injected as in 37 (link) to expand antigen-specific tTregs in vivo. Antigen-specific Tregs were isolated from spleens of the TCR transgenic animals by FACS sorting based on Foxp3 reporter expression. Alternatively, antigen-specific tTregs were expanded in vitro. For in vitro proliferation, Tregs were cultured for three days in the presence of plate bound anti-CD3ε (145–2C11; 2 ug/mL), anti-CD28 (37.51; 2 ug/mL) and IL-2 (100 U/mL). On day 3, cells were split 1:2 and cultured with only IL-2. Tregs were harvested on day 5 and FACS sorted for Foxp3 reporter.
Akkaya B., Oya Y., Akkaya M., Souz J.A., Holstein A.H., Kamenyeva O., Kabat J., Matsumura R., Dorward D.W., Glass D.D, & Shevach E.M. (2019). T regulatory cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells. Nature immunology, 20(2), 218-231.
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