Gentamicin sulfate salt g1264

The minimum inhibitory concentration (MIC) was determined by a modification of the broth dilution technique. Antibiotics were obtained from Sigma-Aldrich (Kanamycin sulfate K1377, Streptomycin sulfate salt S6501, Gentamicin sulfate salt G1264, Neomycin trisulfate salt hydrate N1876, Paromomycin sulfate salt P5057, Hygromycin B H7772, Apramycin sulfate salt A2024). Dilutions of the antibiotic stock were made in sterile distilled water to which equal volume of 2× concentrated LB broth was added. Amount of stock taken was calculated with respect to final diluted volume. Overnight grown culture was added to the broth to achieve 1:100 dilution. After 24-h incubation at 37°C with shaking at 200 rpm, the MIC was inferred as the lowest concentration at which OD₆₀₀ falls below 0.05 with respect to the blank. Eight independently grown replicates were so tested for their MIC.

Polylactide semi-crystalline PLA6202D with M_W of 97 k Da was purchased from NatureWorks^® Ingeo™ (Minnetonka, MN, USA). Chloroform was purchased from VWR (14 Media Village, Leighton Buzzard, LU7 0GA, UK). Gentamicin sulfate salt (G-1264) and polyvinyl alcohol with M_W of 47 k Da, degree of hydrolysis: 98.0–98.8, were supplied by Sigma-Aldrich (St. Louis, MO, USA). Sodium chloride was purchased from Mikrochem (Slovak Republic). Potassium permanganate was purchased from Penta Prague (Czech Republic).