Cd25 b1

Manufactured by Beckman Coulter

Sourced in United States

The CD25 (B1.49.9) is a lab equipment product offered by Beckman Coulter. It is used to detect and measure the expression of the CD25 antigen, which is a marker for activated T cells and B cells. The core function of the CD25 (B1.49.9) is to provide researchers and clinicians with a tool to analyze immune cell activation and function.

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Lab products found in correlation

Facscanto 2 flow cytometry, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Cd14 rmo52, by Beckman Coulter (1 mentions) Cd45ra 2h4, by Beckman Coulter (1 mentions) Cd4 13b8.2, by Beckman Coulter (1 mentions) Cd3 ucht 1, by Beckman Coulter (1 mentions) Cd45 clone j33, by Beckman Coulter (1 mentions) Cd7 8h8.1, by Beckman Coulter (1 mentions) Kaluza analysis software, by Beckman Coulter (1 mentions) Immu 357, by Beckman Coulter (1 mentions)

Spelling variants of this product

Anti-CD25 (B1.49.9; (2 citations) Anti-human CD25-FITC (clone B1.49.9) (2 citations)

2 protocols using cd25 b1

Multiparametric Flow Cytometry Profiling

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Immediately after arrival of the samples, fresh whole blood from each patient was used to analyze immune populations by flow cytometry (100 μL per antibody panel). EDTA was removed from blood by two washes with PBS 1x. Antibody (clone number in parenthesis) used are: Panel 1 Treg-Tfh: CD4 (OKT4), CD25 (B1.49.9, Beckman Coulter), CD127 (A019D5), CXCR5 (J252D4), PD-1(A17188B), CD8 (SK1); Panel 2 Tmem-Teff: CD4 (OKT4), CD8 (RPA-T8), CD45RA (HI100), CXCR3 (G025H7), CCR7 (G043H7), CCR6 (G034E3); Panel 3 Monocytes: CD14 (RMO52, Beckman Coulter), CD3 (UCHT1), CD11c (3.9), CD16 (3G8), HLA-DR (L243), CD56 (5.1H11), CD19 (HIB19); all antibodies from Biolegend^®, San Diego, CA, US, unless otherwise noted. Antibody mixtures were added to the cells and were incubated for 30 min in room temperature. Subsequently, 1 ml of 1x lysing buffer (BD FACS Lysing solution) was added for 10 min. Cells were then washed with FACS buffer (PBS 1x, 2% FBS, 0.02% sodium azide) and immediately run at BD FACSCanto II flow cytometry. FlowJo^® software (FlowJo LLC, Ashland, OR, US) was used for analysis.

Pérez-Sáez M.J., Uffing A., Leon J., Murakami N., Watanabe A., Borges T.J., Sabbisetti V.S., Cureton P., Kenyon V., Keating L., Yee K., Fernandes Satiro C.A., Serena G., Hildebrandt F., Riella C.V., Libermann T.A., Wang M., Pascual J., Bonventre J.V., Cravedi P., Fasano A, & Riella L.V. (2021). Immunological Impact of a Gluten-Free Dairy-Free Diet in Children With Kidney Disease: A Feasibility Study. Frontiers in Immunology, 12, 624821.

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Multiparametric Flow Cytometry of Mucosal Lymphocytes

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PBMCs or lymphocytes isolated from the colon or rectal mucosa were utilized for flow cytometry. Monoclonal antibodies against CD45 (clone J33; Beckman Coulter, Pasadena, CA, USA), CD3 (UCHT1, Beckman Coulter), CD4 (13B8.2; Beckman Coulter), CD8 (B9.11; Beckman Coulter), CD7 (8H8.1; Beckman Coulter), CD25 (B1.49.9; Beckman Coulter), CD30 (HRS4; Beckman Coulter), CD45RA (2H4; Beckman Coulter), CD56 (N901; Beckman Coulter), CD62L (DREG56; Beckman Coulter), CD127 (R34.34; Beckman Coulter), CCR4 (i.e., CD194; L291H4; BioLegend), HLADR (Immu-357; Beckman Coulter), and PD1 (CD279; PD1.3; Beckman Coulter) were employed. The immunostained cells were analyzed using FACScan (Navios flow cytometer, Beckman Coulter) and Kaluza analysis software (version 1.3; Beckman Coulter). Lymphocytes were separated by flow cytometry based on high CD45 antigen expression and forward and side scatter properties. Subsequently, the flow cytometry data were analyzed according to the percentage of cell populations detected in each quadrant on two-dimensional scatterplots. We calculated the percentages of CD4⁺, CD8⁺, CD56⁺, CD7⁺, PD1⁺, CCR4⁺, CD30⁺, and HLADR⁺ cells among CD3⁺ cells. We also assessed the percentages of Treg, CD45RA⁺, and CD62L⁺ cells among CD3⁺CD4⁺ cells and percentages of CD45RA⁺ and CD62L⁺ cells among CD3⁺CD4⁻ cells. In this study, we defined CD3⁺CD4⁺CD25⁺CD127^low/- cells as Treg cells.

Iwamuro M., Takahashi T., Watanabe N., Tanaka T., Inokuchi T., Hiraoka S., Otsuka F, & Okada H. (2022). Enriched CD45RA−CD62L+ central memory T and decreased CD3+CD56+ natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients. International Journal of Immunopathology and Pharmacology, 36, 20587384211051982.

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