Sodium cyanide nacn

Arginine (Arg), aspartate (Asp), citrulline (Cit), glutamate (Glu), histamine (Hist), and taurine (Tau) were obtained from Sigma Aldrich (St Louis, MO). Standards of each amine were prepared at 2 mM concentrations in 18.2 MΩ /cm deionized water (Millipore, Billerica, MA). Subsequent dilutions of each stock solution were made prior to analysis. Naphthalene-2,3-dicarboxaldehyde (NDA) (Invitrogen, Carlsbad, CA) was prepared in acetonitrile (Fisher Scientific, Pittsburgh, PA) to a concentration of 5 mM. Sodium cyanide (NaCN) (Sigma Aldrich) was dissolved in water to a final concentration of 10 mM. Stock solutions of both NDA and NaCN were made weekly and stored at 4°C, protected from light exposure. Stock solutions of sulfobutylether-β-cyclodextrin (SBEC) (Life Technologies, Grand Island NY) were made on a weekly basis to a concentration of 10 mM in deionized water and stored at 4°C. Finally, the background electrolyte (BGE) consisted of 1.4 mM SBEC, 10% by volume HPLC-grade dimethylsulfoxide (Fisher Scientific), and sodium tetraborate (Sigma Aldrich) at a final concentration of 15 mM. Finally, the pH of the BGE was measured using a pH meter and adjusted to 9.2 with 1 M sodium hydroxide (Fisher Scientific).

Murine RAW 264.7 cells (ATCC^® TIB71^™), Dulbecco’s Modified Eagle’s Medium (DMEM), phenol red-free DMEM, fetal bovine serum (FBS), and penicillin/streptomycin antibiotic solution were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). L-carnosine, sodium cyanide (NaCN), anhydrous dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), Trypan blue solution, lipopolysaccharides (LPS), Triton X-100, and bovine serum albumin (BSA) were all supplied by Sigma-Aldrich (St. Louis, MO, USA). Sodium hydroxide (NaOH), hydrochloric acid (HCl), 25 mL polystyrene culture flasks, boric acid, and ethanol (95%) were obtained from Fisher Scientific (Pittsburgh, PA, USA). Interferon-γ (IFN-γ) was supplied by Calbiochem (Gibbstown, NJ, USA). Naphthalene-2,3-dicarboxaldehyde (NDA) was obtained from Invitrogen (Carlsbad, CA, USA). Polyethersulfone (PES) membrane (3K) was purchased from VWR International (West Chester, PA, USA). C-Chip disposable hemocytometer was purchased from Bulldog Bio, Inc. (Portsmouth, NH, USA). All water used was Ultrapure (18.3 MΩ cm) (Milli-Q Synthesis A10, Millipore, Burlington, MA, USA).