Fresh FRAP reagent was prepared by mixing 0.1 M acetate buffer (pH 3.6), 10 mM TPTZ dissolved in 40 M HCL and 20 mM ferric chloride dissolved in distilled water (10:1:1, by vol.). A 0.2 mL sample of wolfberry infusion was added to 4.9 mL of FRAP reagent. After 15 min, A593 was measured with a Shimadzu UV-visible 2550 spectrophotometer and Trolox solution was used to construct the calibration curves. The results are expressed as Trolox equivalent antioxidant capacity (TEAC) mg/g sample dry weight (DW)27 .
Uv visible 2550 spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu UV-visible 2550 spectrophotometer is a laboratory instrument designed to measure the absorption or transmittance of light by a sample over a range of ultraviolet and visible wavelengths. It is capable of performing precise absorbance measurements and can be used for various analytical applications in research and quality control.
Automatically generated - may contain errors
6 protocols using uv visible 2550 spectrophotometer
1
Antioxidant Capacity Evaluation of Wolfberry
2
Wolfberry Antioxidant Capacity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 0.2 mL sample of wolfberry infusion was added to 3.9 mL of 0.1 mM DPPH dissovled in methanol then kept in darkness for 30 min at room temperature. A517 was measured with a Shimadzu UV-visible 2550 spectrophotometer. Trolox solution was used to construct the calibration curves and the results are expressed as TEAC mg/g DW28 (link).
3
Thermoresponsive Polymer Solution Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LCSTs were defined as the temperature corresponding to 50% transmittance of aqueous solution during the heating process. UV-visible 2550 Spectrophotometer (Shimadzu, Japan) at 500 nm with the heating rate as 0.1 °C min−1, and the UV-vis spectra of polymer solution was also obtained through same spectrophotometer from 250 nm to 400 nm. Equivalent amounts of polymers were added to 4.0 mL of aqueous solution of different salts. The mixture was cooled at 4 °C for 10 min to ensure that the polymer was dissolved completely.
4
Absorption Spectroscopy of Algae Filaments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Absorption spectra were recorded with a Shimadzu UV–Visible 2550 spectrophotometer (Kyoto, Japan) at room temperature. Measurements were performed on single layers of adjacent filaments of (30 min) dark-adapted algae, which were carefully placed on a glass slide under the microscope. The diameter of each single filament was 0.26 ± 0.02 mm. Empty spaces between filaments were carefully avoided to provide a homogenous surface for the spectrophotometer beam.
5
Organic Dye Degradation Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Of the samples, 5 mg was re-dispersed in 10 mL of the organic dyes (concentration 10 mg/L) and the mixed solution was stored in the dark for 45 min with gentle stirring. The reaction solution was sampled every 15-min intervals at room temperature; 2 mL solution was sampled and centrifuged to remove the adsorbents, and the corresponding UV-visible spectra were recorded to monitor the progress of the degradation of organic dyes by a Shimadzu 2550 UV-visible spectrophotometer.
6
Characterization of Nanoparticles by FT-IR, TEM, and DLS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Fourier transform-infrared (FT-IR) spectrum of particles was recorded using a Nicolet 5700 FT-IR spectrometer (Thermo Fisher Scientific, Inc.), followed by drying. A Hitachi H-600 transmission electron microscope (TEM; Hitachi, Ltd., Tokyo, Japan) was used to acquire images of particles, whereas particles size and zeta potential were characterized by dynamic light scattering using Zetasizer Nano ZS (Malvern Instruments Ltd., Malvern, UK). A 2550 UV-visible spectrophotometer (Shimadzu Corporation, Tokyo, Japan) was used to determine the surface plasmon resonance (SPR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!