Data were collected as reported in [22 (link)] and as recounted briefly here. The CEUS images were taken from the chorioallantoic membrane (CAM) of chicken embryos. This model is attractive due to the long microbubble recirculation times, small bulk tissue motions, and the ability to directly compare the US images with high resolution optical microscopy images of the vasculature. A bolus injection of microbubbles (Lumason, Bracco Diagnostics Inc., Monroe Township, NJ) at 1.8 × 109 microbubbles per milliliter, and imaging acquisitions were performed at the microbubble concentration plateau after the injection.
CEUS images were obtained with a Verasonics Vantage 256 ultrasound system (Verasonics Inc., Kirkland, WA, USA) with a 25 MHz linear array transducer (L35–16vX, Verasonics Inc.). Ultrafast plane wave imaging (15 angles, − 7° to 7°) was performed at 500 compounded frames per second, with 5 V transmit excitation. At each location, 5 successive acquisitions of 720 frames each (total acquisition length 3600 frames over 7.2 seconds) were captured. The IQ data were stored and post-processed with custom MATLAB scripts on a standard desktop computer (4 cores at 2.8 GHz, 16 GB RAM).
CEUS images were obtained with a Verasonics Vantage 256 ultrasound system (Verasonics Inc., Kirkland, WA, USA) with a 25 MHz linear array transducer (L35–16vX, Verasonics Inc.). Ultrafast plane wave imaging (15 angles, − 7° to 7°) was performed at 500 compounded frames per second, with 5 V transmit excitation. At each location, 5 successive acquisitions of 720 frames each (total acquisition length 3600 frames over 7.2 seconds) were captured. The IQ data were stored and post-processed with custom MATLAB scripts on a standard desktop computer (4 cores at 2.8 GHz, 16 GB RAM).