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Fgf 21 elisa kit

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The FGF-21 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of FGF-21 (Fibroblast Growth Factor 21) levels in biological samples. The kit uses a specific antibody coated on a microplate to capture FGF-21 from the sample, and a second antibody conjugated with an enzyme is used for detection and quantification.

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Lab products found in correlation

Dy1119, by R&D Systems (2 mentions) Mouse leptin elisa kit, by R&D Systems (2 mentions) Elm resistin, by RayBiotech (2 mentions) 47 adpms e01, by ALPCO (2 mentions) Insulin elisa kit, by Merck Group (1 mentions) Free glycerol reagent, by Merck Group (1 mentions) Glycerol, by Merck Group (1 mentions)

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FGF-21 (2 citations)

3 protocols using fgf 21 elisa kit

1

Adipokine Profiling in Mouse Serum

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High molecular weight (HMW) adiponectin levels and total adiponectin levels in mouse serum were determined using ELISA (47-ADPMS-E01, ALPCO; and DY1119, R&D Systems), according to the manufacture’s protocol. For serum insulin measurement, mice were i.p. injected with glucose (1.5 g/kg) after 6 hours of fasting, sera were collected at 0, 15, and 30 min after glucose injection, and serum insulin levels were measured by ELISA (90080; Crystal Chem) according to the manufacture’s protocol. Leptin levels in the mouse serum and 3T3-L1 medium were measured by the mouse leptin ELISA kit (DY498–05, R&D Systems). Resistin levels were measured using the resistin ELISA kit (ELM-Resistin, RayBiotech, Inc.). Serum FGF-21 levels were determined using the FGF-21 ELISA kit (KE10042, Proteintech).
Kuramoto K., Kim Y.J., Hong J.H, & He C. (2021). The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding. Cell reports, 35(8), 109184.
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2

Metabolic Biomarkers in High-Fat Diet Mice

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After a 12-week HFD regimen, mice were fasted overnight (16 hours) beginning at 5 p.m. Blood samples were obtained for glucose and insulin measurements. Plasma insulin levels were determined using an insulin ELISA kit (Millipore). Nonfasting serum FGF21 levels were measured by an FGF21 ELISA kit (Proteintech, KE10042) according to the manufacturer’s instructions. Nonfasting serum GDF15 levels were determined using a GDF15 ELISA kit (ImmunoDiagnostics, 32980). Liver tissue (50 mg) was homogenized, and centrifuged supernatants were harvested. The TG levels were determined with the Free glycerol Reagent (Sigma-Aldrich, F6428) using glycerol (Sigma-Aldrich, G7793) as the standard for calculation (10 (link)).
Guo Q., Xu Z., Zhou D., Fu T., Wang W., Sun W., Xiao L., Liu L., Ding C., Yin Y., Zhou Z., Sun Z., Zhu Y., Zhou W., Jia Y., Xue J., Chen Y., Chen X.W., Piao H.L., Lu B, & Gan Z. (2022). Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4. Science Advances, 8(30), eabo0340.
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3

Adipokine Profiling in Mouse Serum

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High molecular weight (HMW) adiponectin levels and total adiponectin levels in mouse serum were determined using ELISA (47-ADPMS-E01, ALPCO; and DY1119, R&D Systems), according to the manufacture’s protocol. For serum insulin measurement, mice were i.p. injected with glucose (1.5 g/kg) after 6 hours of fasting, sera were collected at 0, 15, and 30 min after glucose injection, and serum insulin levels were measured by ELISA (90080; Crystal Chem) according to the manufacture’s protocol. Leptin levels in the mouse serum and 3T3-L1 medium were measured by the mouse leptin ELISA kit (DY498–05, R&D Systems). Resistin levels were measured using the resistin ELISA kit (ELM-Resistin, RayBiotech, Inc.). Serum FGF-21 levels were determined using the FGF-21 ELISA kit (KE10042, Proteintech).
Kuramoto K., Kim Y.J., Hong J.H, & He C. (2021). The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding. Cell reports, 35(8), 109184.
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