The aqueous extract of Salvia plebeia R. (FIE-SP, SPW) was provided by FromBio (Kyounggido, Korea). Briefly, dried leaves of SP were cultivated in Gyeongsangbuk-do, Korea, soaked with water, and the mixture was then heated for 8 h at 80–85 °C, filtered, and dried by a spray dryer. Dulbecco’s modified eagle medium (DMEM), RPMI-1640, fetal bovine serum (FBS), and penicillin were obtained from Gibco BRL (Grand Island, NY, USA). The CyQUANT™ lactate dehydrogenase (LDH) cytotoxicity assay kit and the immunoglobulin ELISA assay kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Concanavalin A, IL-1β, IL-2, IL-4, IL-10, IL-12, IFN-γ, and TNF-α ELISA assay kits were purchased from R&D system (Mckinley Place, NE, USA). Mouse CD45 PerCP, mouse CD8a FITC, mouse CD4 PE, mouse major histocompatibility complex (MHCI), FITC, and mouse MHCII PE antibody were purchased from eBioscience (San Diego, CA, USA).
Concanavalin a (cona)
Manufactured by R&D Systems
Sourced in United States, Japan
Concanavalin A is a lectin protein derived from the jack bean (Canavalia ensiformis). It has the ability to agglutinate red blood cells and bind to specific carbohydrate groups on the cell surface. Concanavalin A is commonly used in biochemical and cell biology research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Interleukin 2 (il 2), by R&D Systems (1 mentions)
Cyquant lactate dehydrogenase ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
Mouse cd45 percp, by Thermo Fisher Scientific (1 mentions)
Mouse cd8a fitc, by Thermo Fisher Scientific (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Recombinant human il 2, by R&D Systems (1 mentions)
Rpmi 1640, by Fujifilm (1 mentions)
Cd4 l3t4 microbeads, by Miltenyi Biotec (1 mentions)
Lympholyte m, by Cedarlane (1 mentions)
2 protocols using concanavalin a (cona)
1
Salvia plebeia R. Extract Immunomodulatory Effects
2
Adoptive Transfer of Activated CD4+ T Cells
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Total lymphoid cells were recovered from the splenocytes of BALB/c mice by lympholyte-M (Cedarlane Lab., Ontario, Canada). Then, cell suspension was treated with HLB solution (IBL) for hemolysis. Total lymphoid cells (2 × 106 cells/mL) were cultured in RPMI-1640 (WAKO, Japan) supplemented with 10% FBS (Gibco Life Technologies), Concanavalin A (4 μg/mL), and recombinant human IL-2 (10 ng/mL, R&D systems, Minneapolis, MN, USA) for 3 days. After incubation, CD4+ T cells were isolated by MACS separation systems with CD4 (L3T4) MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Activated CD4+ T cells (2 × 105 cells) from BALB/c mice were intravenously injected into SCID mice (day 0). At day 17, the diarrhea score for stool consistency was graded on a scale of 1–4 (1, normal; 2, pasty and formed; 3, pasty and unformed; 4, diarrhea).
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