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Glutathione reductase

Manufactured by Cayman Chemical
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Glutathione reductase is an enzyme that catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell.

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Lab products found in correlation

Colorimetric assay kit, by Cayman Chemical (1 mentions) Glutathione peroxidase, by Cayman Chemical (1 mentions) Catalase, by Cayman Chemical (1 mentions) Nadph, by Cayman Chemical (1 mentions)

Close spelling variants of this product

Glutathione Reductase Assay Kit (17 citations) Cayman’s Glutathione Reductase Assay Kit (2 citations)

2 protocols using glutathione reductase

1

Antioxidant Enzyme Activity Assay

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Muscle lysates were used to determine total antioxidant capacity (TAC) (Cayman, MI, USA, Cat# 709001), and the enzymatic activities of catalase (Cayman, MI, USA, Cat# 707002), glutathione peroxidase (Cayman, MI, USA, Cat# 703102), and glutathione reductase (Cayman, MI, USA, Cat# 703202) according to the manufacturer’s instructions for each assay kit. Enzymatic activity was normalized by the quantity of protein (mg) used in each essay. Superoxide dismutase activity was also assessed using a colorimetric assay kit (Cayman, MI, USA, Cat# 706002), but the interference of the reagents used in the homogenization of muscle samples prevented the acquisition of reliable data. Therefore, SOD activity is not reported herein.
Mesquita P.H., Lamb D.A., Godwin J.S., Osburn S.C., Ruple B.A., Moore J.H., Vann C.G., Huggins K.W., Fruge A.D., Young K.C., Kavazis A.N, & Roberts M.D. (2021). Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults. Antioxidants, 10(3), 350.
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2

Assaying Thiol Content in K. brevis

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Homogenate of K. brevis (275 µL, 0.3–0.5 mg / mL) was mixed with NADPH (100 µL, 1 mg / mL), glutathione reductase (3 µL, 10 µM, Cayman Chemical) and Ellman’s reagent (25 µL, 4 mg / mL). Absorbance was measured immediately every 10 min at 410 nm at room temperature for up to 30 min. Background absorbance of homogenate alone was subtracted from each sample. Ellman’s reagent in reaction buffer served as a control. Samples were quantitated against cysteine standards (0, 0.3, 0.6 and 1.2 mM) prepared in reaction buffer. Samples and cysteine standards were analyzed in triplicate. Thiol content was normalized to total protein in the homogenate.
Chen W., Colon R., Louda J.W., del Rey F.R., Durham M, & Rein K.S. (2018). Brevetoxin (PbTx-2) influences the redox status and NPQ of Karenia brevis by way of thioredoxin reductase. Harmful algae, 71, 29-39.
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