Ndufs1

Manufactured by GeneTex

NDUFS1 is a nuclear-encoded subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I), which is the largest of the respiratory chain complexes. It is an essential component for the enzyme's catalytic activity and structural integrity.

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Lab products found in correlation

Bis tris gradient gel, by Thermo Fisher Scientific (1 mentions) Vdac1, by Abcam (1 mentions) Uqcrfs1, by GeneTex (1 mentions) Peroxidase conjugated anti mouse and anti rabbit iggs, by Thermo Fisher Scientific (1 mentions) Turbo gfp, by OriGene (1 mentions) Cox5b, by Santa Cruz Biotechnology (1 mentions) Ecl prime western blotting detection reagent, by Cytiva (1 mentions) Chemidoc mp imager, by Bio-Rad (1 mentions) β actin, by Merck Group (1 mentions) Image lab, by Bio-Rad (1 mentions) Ndufb8, by Abcam (1 mentions) Core 2, by Abcam (1 mentions) Cox5a, by Abcam (1 mentions) Total oxphos human wb antibody cocktail, by Abcam (1 mentions)

3 protocols using ndufs1

Mitochondrial Protein Analysis by SDS-PAGE

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Mitochondrial fractions were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (PAGE). Thirty micrograms of mitochondrial protein was separated on 4%–12% Bis-Tris gradient gels (Life Technologies, Carlsbad, CA) and transferred to polyvinylidene fluoride membranes as described.^{27 (link)} The membranes were incubated with primary antibodies against IBA57 (Sigma, St. Louis, MO), VDAC1 (Abcam, Cambridge, UK), LA (Calbiochem, San Diego, CA), NDUFS1, NDUFS8, NDUFV1, SDHB, and UQCRFS1 (all from GeneTex, Irvine, CA). Protein levels of IBA57 and ISC were quantified with ImageJ software (National Institutes of Health, Bethesda, MD).

Ishiyama A., Sakai C., Matsushima Y., Noguchi S., Mitsuhashi S., Endo Y., Hayashi Y.K., Saito Y., Nakagawa E., Komaki H., Sugai K., Sasaki M., Sato N., Nonaka I., Goto Y.I, & Nishino I. (2017). IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy. Neurology: Genetics, 3(5), e184.

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Comprehensive Western Blot Analysis

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Western blot was performed using primary antibodies raised against COX7A2L (ProteinTech), Myc (Origene), turbo-GFP (Origene), HA (Roche), β-actin (Sigma), and against the following human OXPHOS subunits: NDUFS1 (GeneTex); NDUFA9, NDUFB8, CORE2, RISP, CYC1, UQCRB, UQCRQ, COX1, COX4, COX5A, COX6C, SDHA, SDHB (Mitosciences); and COX5B (Santa Cruz). Peroxidase-conjugated anti-mouse and anti-rabbit IgGs were used as secondary antibodies (Molecular Probes). Immunoreactive bands were detected with an ECL prime Western Blotting Detection Reagent (Amersham) in a ChemiDoc™ MP Imager (Biorad). Optical densities of the immunoreactive bands were measured using the ImageLab™ (Biorad) and ImageJ analysis softwares.

Pérez-Pérez R., Lobo-Jarne T., Milenkovic D., Mourier A., Bratic A., García-Bartolomé A., Fernández-Vizarra E., Cadenas S., Delmiro A., García-Consuegra I., Arenas J., Martín M.A., Larsson N.G, & Ugalde C. (2016). COX7A2L is a mitochondrial complex III-binding protein that stabilizes the III2+IV supercomplex without affecting respirasome formation. Cell reports, 16(9), 2387-2398.

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OXPHOS Protein Expression Analysis

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Cells were treated with MO as indicated concentrations for 48 h. The protein expression level of OXPHOS complexes were determined by western blot following the procedures described previously [11 (link)], without heating up the samples. The total OXPHOS human WB antibody cocktail was purchased from Abcam (ab110411). NDUFS1 was purchased from Genetex (GTX113787).

Kuo T.T., Lin L.C., Chang H.Y., Chiang P.J., Wu H.Y., Chen T.Y., Hsia S.M, & Huang T.C. (2022). Quantitative Proteome Analysis Reveals Melissa officinalis Extract Targets Mitochondrial Respiration in Colon Cancer Cells. Molecules, 27(14), 4533.

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