Dulbecco’s modified Eagle medium (DMEM; #12800-017) was purchased from Gibco (Rockville, MD, USA). Hyclone™ Antibiotic Antimycotic solution (#SV30079.01) and fetal bovine serum (FBS; FetalClone III™, #SH30109.03) were procured from GE Healthcare Life Sciences (Chicago, IL, USA). Primary antibodies against phospho-AKT (#GTX50128), PDGFR-β (#GTX61115), phospho-PDGFR-β (#GTX61797), and HIF-1α (#GTX127309), as well as horseradish peroxidase (HRP)-conjugated secondary antibodies against mouse (#GTX213112-01) and rabbit (#GTX213110-01) immunoglobulin G (IgG) were purchased from GeneTex (Irvine, CA, USA). Antibodies detecting β-actin (#66009-1-Ig), phospho-extracellular signal-regulated kinase 1/2 (ERK1/2; #05-797R), and phospho-mammalian target of rapamycin (mTOR; #ab109268) were respectively obtained from Proteintech (Rosemont, IL, USA), Millipore (Bedford, MA, USA), and Abcam (Cambridge, MA, USA). Recombinant human PDGF-BB (#100-14B) and AG-1295 (#14529), a PDGFR-β inhibitor, were respectively bought from PeproTech (Rocky Hill, NJ, USA) and Cayman Chemical (Ann Arbor, MI, USA). Cobalt dichloride (#C8661) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
Cobalt dichloride
Manufactured by Merck Group
Sourced in United States
Cobalt dichloride is an inorganic compound with the chemical formula CoCl2. It is a blue crystalline solid that is soluble in water and other polar solvents. Cobalt dichloride is commonly used as a desiccant, moisture indicator, and in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated secondary antibody, by GeneTex (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Recombinant human pdgf bb 100 14b, by Thermo Fisher Scientific (1 mentions)
Quisinostat, by MedChemExpress (1 mentions)
Doxycycline dox, by Sangon (1 mentions)
Sch772984, by Selleck Chemicals (1 mentions)
Chloroquine, by Sangon (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Hoechst 33422, by Thermo Fisher Scientific (1 mentions)
Oxalyl chloride, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
3 protocols using cobalt dichloride
1
PDGFR-β Signaling Pathway Analysis
2
Doxycycline-Induced Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Doxycycline (DOX; Sangon Biotech) was prepared in PBS at a stock concentration of 10 mg/ml. Cells were incubated with 1 μg/ml DOX for 0, 6, 12, 24, and 48 hours, and then, the samples were collected for western blot (WB). To exclude the potential effects of DOX on cytotoxicity, DOX was used at the same concentration to treat parental SKOV3 and vector control SKOV3-Vec cells for 48 hours. All cells were harvested for different tests after treatment.
Cells treated with either the ERK inhibitor SCH772984 (2 μM Selleck) or AKT inhibitor GSK2110183 (10 nM; MedChem Express) for 8 hours were also used for western blot (WB). To inhibit the activation of HDAC4, the HDACs inhibitor Quisinostat (100nM, MedChem Express) was applied to cells for 24 hours. DMSO-treated cells were used as controls. Cells were treated with chloroquine (25 μM; Sangon Biotech) for 20 hours to compromise autophagic degradation. The IC50 values for cisplatin were determined with an MTT assay at concentrations ranging from 0.0256 μM-10,000 μM. To mimic a hypoxic environment, 100 μM cobalt dichloride (Sigma) was used to treat cells for 6 hours.
Cells treated with either the ERK inhibitor SCH772984 (2 μM Selleck) or AKT inhibitor GSK2110183 (10 nM; MedChem Express) for 8 hours were also used for western blot (WB). To inhibit the activation of HDAC4, the HDACs inhibitor Quisinostat (100nM, MedChem Express) was applied to cells for 24 hours. DMSO-treated cells were used as controls. Cells were treated with chloroquine (25 μM; Sangon Biotech) for 20 hours to compromise autophagic degradation. The IC50 values for cisplatin were determined with an MTT assay at concentrations ranging from 0.0256 μM-10,000 μM. To mimic a hypoxic environment, 100 μM cobalt dichloride (Sigma) was used to treat cells for 6 hours.
3
Fluorescent Probes for Cell Viability Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zirconium chloride anhydrous (ZrCl 4 ), protoporphyrin IX (PpIX), acetic acid, cobalt dichloride, dichloromethane (CH 2 Cl 2 ), oxalyl chloride, 2',7'-dichlorofluorescein diacetate (DCFH-DA), dimethylformamide (DMF), tetrahydrofuran (THF), ethanol, methanol were purchased from Sigma-Aldrich. 2'-aminoterphenyl-4,4''-dicarboxylic acid (TPDC-NH 2 ) was synthesized according to the reported reference. 41 Fetal bovine serum (FBS), phosphate buffered saline (PBS), Dulbecco's modified Eagle medium (DMEM), penicillin streptomycin 10,000 U/mL (PS), trypsin, calcein acetoxymethyl ester (calcein-AM), propidium iodide (PI), and Hoechst 33422 were ordered from Invitrogen. Low viscosity embedding media spur's kit was purchased from Electron Microscopy Sciences. Unless otherwise noted, all chemicals were used without further purification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!