Field emission scanning electron microscopy (FESEM) analysis was studied by a MAIA3 LMH scanning electron microscope (Waltham, MA, USA). X-ray photoelectron spectroscopy (XPS) was performed on an ESCALAB 250Xi spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) using a monochromatized Al Kα source (hν = 1486.6 eV). Element analysis for C, H, N, and O was determined using a Euro EA3000 analyzer (Milan, Italy). Fourier transform infrared spectroscopy (FT-IR) spectra were recorded using a Bruker VERTEX70 spectrometer (Waltham, MA, USA). Analytes quantification was carried out on a Shimadzu LC 2040C 3D ultra-high performance liquid chromatography (UPLC) system (Tokyo, Japan) with a photodiode array (PDA) detector and 40 μL of the sample loop. The separation was carried out using a C18 column (75 mm × 2.0 mm i.d.) with a 2.2 μm particle size from Shimadzu (Tokyo, Japan) at 30 °C. The mobile phase consisted of (A) 25 mM H3PO4 aqueous solution (pH was adjusted to 3.0 with trimethylamine) and (B) ACN. The gradient program was as follows: 0–9 min, 10.5%–14% B; 9–10 min, 14%–10.5% B; and 10–13 min, 10.5% B. The flow rate was maintained at 0.3 mL/min. The wavelength number was set as 278 nm and the injection volume was 10 μL.
Lc 2040c 3d
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu LC-2040C 3D is a high-performance liquid chromatography (HPLC) system. It is designed for efficient separation, identification, and quantification of chemical compounds in a variety of sample types. The LC-2040C 3D features a compact and modular design, allowing for easy integration into laboratory workflows.
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3 protocols using lc 2040c 3d
1
Comprehensive Analytical Techniques for Material Characterization
2
Quantification of Capmatinib and Warfarin by HPLC
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The separation and quantification were carried out using the Shimadzu LC-2040C 3D plus (Tokyo, Japan) HPLC system, equipped with either a fluorescence detector (FLD), RF-20A, or a photodiode array detector (PDA). The FLD was set at the excitation and emission wavelengths of 405 nm and 495 nm for the capmatinib, respectively, and 310 and 390 nm for the warfarin, respectively. The PDA detector monitored the capmatinib and naproxen at 270 and 232 nm, respectively. Data acquisition and integration were attained using LabSolutions software, Shimadzu Corporation, Kyoto, Japan (version 6.87 SP1).
3
HPLC Determination of Warfarin and Metabolites
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HPLC used was Shimadzu LC-2040C 3D plus HPLC system (Japan), which was equipped with a fluorescence detector (FLD), RF-20A. For warfarin, its metabolites, and the internal standard naproxen, the FLD was set at excitation and emission wavelengths; 310 nm and 390 nm, respectively. Data processing was performed on LabSolutions software version 6.87. Separation was achieved using Kinetex® pentafluorophenyl (PFP) column (100 × 3.0, 2.6 μm) with a mobile phase mixture consisting of 15 mM ammonium acetate buffer at pH 7 and methanol in a ratio of 60:40 (v/v) and delivered in isocratic mode at a flow rate of 0.7 mL/min. The chromatographic conditions are shown in Table 1 .Table 1
Optimized parameters of the proposed HPLC method.
| Column | Kinetex® PFP column (100 × 3.0 mm, 2.6 μm) |
|---|---|
| Flow rate | 0.7 mL/min |
| Injection volume | 5 μL |
| Mobile phase and flow rate | 60:40 v/v (15 mM ammonium acetate buffer pH 7: methanol) |
| Temperature | 40 °C |
| Wavelengths (λ ex, λ em) | 310, 390 nm |
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