Dp11 camera

Fifteen micron sections of optimal cutting temperature compound-embedded (Tissue-Tek; Sakura Finetek) tumors were immunostained with anti-CD31 antibodies to assess microvessel density (MVD). To quantify MVD, the number of immunostained vessels in ten 0.159 mm² fields at a magnification of × 100 from ten randomly selected tumors in each group was counted. Five micrometer sections of paraffin-embedded tumor tissue were immunostained with anti-p-Ser¹⁰ histone H3 or cleaved PARP antibodies to assess tumor-cell proliferation and apoptosis, respectively, based on the percentage of p-Ser¹⁰ histone H3-positive and cleaved PARP-positive cells per ≥500 cell region, respectively. Three tumors per treatment and four regions per tumor were analyzed for tumor cell proliferation and apoptosis. Induction of apoptosis was defined as a two-fold or greater increase in the proportion of cells identified as cleaved PARP-positive in tumors from regorafenib-treated mice compared with tumors from vehicle-treated animals. Images were recorded using an Olympus BX60 microscope equipped with an Olympus DP11 camera. All experiments were performed in triplicate.
Tumor necrosis was assessed by microscopic examination of hematoxylin and eosin (H&E)-stained tumor sections, with ten random fields examined at a × 100 magnification. Tumor necrosis was only qualitatively assessed.

Live specimens were taken from the caves and brought to the laboratory where they were placed in a terrarium with cave substrate. Before being photographed, the specimens were placed in a Petri dish with cave substrate and left for a few minutes in a refrigerator. Photographs were taken with an Olympus DP11 camera coupled to an Olympus SZH10 stereomicroscope. The insects were kept in the dark and only the cold light of the transilluminator was used.