Anti s100a4 clone d9f9d

Manufactured by Cell Signaling Technology

Sourced in Netherlands

Anti-S100A4 (clone D9F9D) is a monoclonal antibody that recognizes the S100A4 protein. S100A4 is a calcium-binding protein involved in various cellular processes. This antibody can be used for the detection of S100A4 in biological samples.

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Lab products found in correlation

Histone h1 clone ae 4, by Bio-Rad (2 mentions) Nuclear cytosol fractionation kit, by Cambridge Bioscience (2 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-S100A4 (7 citations)

2 protocols using anti s100a4 clone d9f9d

Protein Isolation and Analysis from CD34+ Cells and AML Blasts

Cited 1 time

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Nuclear and cytoplasmic proteins were isolated from
>5×10⁶ fresh/frozen CD34⁺ cells and AML
blasts using the Nuclear/Cytosol Fractionation Kit (Cambridge Bioscience, U.K.)
following manufacturer’s instructions. A fraction of AML cells were also
lysed in Trizol^® for comparative mRNA analysis (Supplemental Methods)
[18 ].
Western blotting was carried out as previously described [19 (link)] with the following antibodies:
anti-S100A4 (clone D9F9D, Cell Signaling Technologies (CST), Netherlands),
Histone H1 (clone AE-4, AbD Serotec, U.K.), H3 (CST) and
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (6C5, Santa Cruz Biotechnology,
Heidelberg, Germany).
Detailed MS proteomic methods and data analysis are shown in Supplemental Methods. The
MS proteomics data have been deposited to the ProteomeXchange Consortium
(http://proteomecentral.proteomexchange.org) via the PRIDE
partner repository with the data set identifier PXD002799.

Alanazi B., Munje C.R., Rastogi N., Williamson A.J., Taylor S., Hole P.S., Hodges M., Doyle M., Baker S., Gilkes A.F., Knapper S., Pierce A., Whetton A.D., Darley R.L, & Tonks A. (2019). Integrated nuclear proteomics and transcriptomics identifies S100A4 as a therapeutic target in acute myeloid leukemia. Leukemia, 34(2), 427-440.

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Protein Isolation and Analysis from CD34+ and AML Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nuclear and cytoplasmic proteins were isolated from >5 × 10⁶ fresh/frozen CD34⁺ cells and AML blasts using the Nuclear/Cytosol Fractionation Kit (Cambridge Bioscience, U.K.) following manufacturer’s instructions. A fraction of AML cells were also lysed in Trizol® for comparative mRNA analysis (Supplementary Methods) [18 (link)].
Western blotting was carried out as previously described [19 (link)] with the following antibodies: anti-S100A4 (clone D9F9D, Cell Signaling Technologies (CST), Netherlands), Histone H1 (clone AE-4, AbD Serotec, U.K.), H3 (CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (6C5, Santa Cruz Biotechnology, Heidelberg, Germany).
Detailed MS proteomic methods and data analysis are shown in Supplementary Methods. The MS proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the data set identifier PXD002799.

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