Neutralizing antibodies against CRP, MIP-1α, MCP-1, RANKL, and control IgG, and recombinant human CRP, M-CSF, RANKL, and SAP were purchased from R&D Systems (Minneapolis, MN). The p38 MAPK-specific inhibitor was purchased from Axon Medchem BV (Groningen, Netherlands). Except where specified, antibodies for western blotting analysis were purchased from Cell Signaling Technology, MA.
Recombinant human crp
Manufactured by R&D Systems
Recombinant human CRP (Catalog # 1707-CR) is a 231 amino acid protein produced in a mammalian cell expression system. It is a member of the pentraxin family of proteins and functions as an acute-phase reactant during inflammation.
Automatically generated - may contain errors
Lab products found in correlation
Control igg, by R&D Systems (1 mentions)
Mip 1α, by R&D Systems (1 mentions)
M csf, by R&D Systems (1 mentions)
Mcp 1, by R&D Systems (1 mentions)
Rankl, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Recombinant human il 6, by Cell Signaling Technology (1 mentions)
Qgp 1, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
T75 flask, by Corning (1 mentions)
2 protocols using recombinant human crp
1
Neutralizing Antibodies Characterization
2
pNEN Cell Lines and Inflammatory Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human metastasized adherent pNEN cell line BON1 was obtained from Dr M Kidd, Yale University School of Medicine, as a gift and has been authenticated by STRS analysis. BON1 cells were cultured as a monolayer in T-75 flasks (Corning) in RPMI 1640:Ham’s F-12 medium in a 1:1 volume ratio (Invitrogen) supplemented with 10% fetal bovine serum (Life Technologies) and penicillin/streptomycin (100 IU/mL) at 37°C with 5% CO2 as previously described (36 (link)).
The human adherent pNEN cell line QGP1 was purchased from the Japanese Cancer Research Resources Bank (JCRB), which is part of the National Institutes of Biomedical Innovation, Health and Nutrition and was cultivated as a monolayer in T-75 flasks in RPMI 1640 supplemented with 10% fetal bovine serum (Life Technologies) and penicillin/streptomycin (100 IU/mL) at 37°C with 5% CO2.
To investigate the role of CRP as well as inflammation in pNEN cell proliferation and metastasis, BON1 and QGP1 cells were treated with the pro-inflammatory cytokine IL-6 (recombinant human IL-6, 25 ng/ml, Cell Signaling) or recombinant human CRP (20 µg/mL) (R&D Systems) for 48 h.
The human adherent pNEN cell line QGP1 was purchased from the Japanese Cancer Research Resources Bank (JCRB), which is part of the National Institutes of Biomedical Innovation, Health and Nutrition and was cultivated as a monolayer in T-75 flasks in RPMI 1640 supplemented with 10% fetal bovine serum (Life Technologies) and penicillin/streptomycin (100 IU/mL) at 37°C with 5% CO2.
To investigate the role of CRP as well as inflammation in pNEN cell proliferation and metastasis, BON1 and QGP1 cells were treated with the pro-inflammatory cytokine IL-6 (recombinant human IL-6, 25 ng/ml, Cell Signaling) or recombinant human CRP (20 µg/mL) (R&D Systems) for 48 h.
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