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Superfrost plus vwr micro slides

Manufactured by Avantor

Superfrost Plus VWR Micro Slides are microscope slides designed for a variety of scientific applications. They feature a positively charged surface that enhances the adhesion of biological samples.

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2 protocols using superfrost plus vwr micro slides

1

Cryosectioning of Human Liver Samples

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Samples of human livers were purchased from BioIVT or shared by collaborators at MGH (Hannah K. Drescher and Lea M. Bartsch). Informed consent was obtained by BioIVT or MGH from all human research participants. Sample ID numbers and donor information are obtained from either BioIVT or MGH and reported in Supplementary Table 1. Frozen samples were embedded in OCT compound (Tissue-Tek, 4583), re-frozen on dry ice, and stored at −80 °C. Embedded samples were sectioned using the cryostat at the W.M. Keck Microscopy Facility, MIT. Sectioning was performed at −21 °C to generate 10 μm-thick slices that were then placed on a Superfrost Plus VWR Micro Slides (VWR, 48311-703) and stored at −20 °C. Images of hematoxylin and eosin (H&E)-stained liver tissue were obtained from BioIVT.
IRB 1999P004983 and IRB 2019P001245, COUHES E3272 and COUHES E3665.
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2

Synthesis and Characterization of Gold Nanoparticles

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Gold(III) chloride trihydrate (>99.99%), sodium borohydride (reagent grade), tetraoctylammonium bromide (98%), didodecyldimethylammonium bromide (98%), tetradecanethiol (reagent grade), chloroform (HPLC grade), tetradecane (≥99%), hexadecane (99%), 1-tetradecene (≥97.0%), 1-tetradecyne (≥97.0%), 1-phenyltridecane (99%), isopropyl myristate (98%), paraffin wax (mp 58-62°C), and toluene (HPLC grade) were purchased from Page 6 of 40 ACS Paragon Plus Environment Langmuir Sigma-Aldrich Canada. 1-Octadecene (≥99.5%) was purchased from Supelco. Anhydrous ethanol was purchased from Commercial Alcohols. All distilled water used was deionized to a resistivity of 18.2 MΩ cm with a Millipore Milli-Q system. Four hundred mesh copper TEM substrates coated with carbon and Formvar were purchased from Ladd Research Industries (Vermont). Superfrost® Plus VWR Micro Slides were used to deposit AuNP-oil films for analysis with UV-vis spectroscopy.
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