Antibody to gapdh

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Antibody to GAPDH is a laboratory reagent used to detect the presence and quantity of the GAPDH protein in biological samples. GAPDH is a commonly used reference protein for various analytical techniques. This antibody can be used to measure GAPDH levels in cell lysates, tissue extracts, or other sample types.

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GAPDH (3335 citations) GAPDH antibody (213 citations) Mouse monoclonal antibody to GAPDH (3 citations) Rabbit polyclonal antibody to GAPDH (2 citations)

8 protocols using antibody to gapdh

Rosuvastatin Inhibits Tumor Cell Invasion

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RT was dissolved in dimethyl sulfoxide (DMSO) as a stock solution and stored at −20 °C until needed. The final concentration of DMSO did not exceed 0.1% throughout the study (this concentration was found to have no effect on cell invasion or cell growth). The chemical structure of RT is shown in Figure 1. The compound was prepared at concentrations of 80 ng/mL and 160 ng/mL. Primary antibodies for vimentin (CST, Boston, MA, USA), STAT3 (CST, Boston, MA, USA), p-STAT3 (CST, Boston, MA, USA) and Nrf2 (CST, Boston, MA, USA) were detected by Western blot. Antibody to GAPDH was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cell Counting Kit-8 (CCK-8) was purchased from Good Laboratory Practice Bioscience (Glpbio, Montclair, CA, USA). IRDye TM800 conjugated second antibody was obtained from Rockland (Gilbertsville, PA, USA). BCA Protein Assay kit was purchased from Beyotime (Beyotime, Shanghai, China).

Yu B., Liang J., Li X., Liu L., Yao J., Chen X, & Chen R. (2022). Renieramycin T Inhibits Melanoma B16F10 Cell Metastasis and Invasion via Regulating Nrf2 and STAT3 Signaling Pathways. Molecules, 27(16), 5337.

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Immunoblotting and Immunofluorescence Analysis

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Antibodies to human HS3ST3B and Nrp1 were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies to phospho-Akt(S⁴⁷³), total Akt, phospho-Src(Y⁴¹⁶), total c-Src, and secondary antibodies conjugated to HRP were from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies conjugated to Alexa-488 (green fluorescent dye) and to Alexa-568 (red fluorescent dye) were from Thermo Fisher Scientific (Waltham, MA, USA). Recombinant HSV-1 gD protein and antibody to HSV-1 gD (clone 1.3) were obtained from Antibodies-online (Aachen, Germany) and Abcam (Cambridge, UK), respectively. Antibody to GAPDH was from Santa Cruz (Santa Cruz, CA, USA). Other chemicals were from Sigma-Aldrich (Darmstadt, Germany) unless otherwise specified.

Hellec C., Diawara M., Carpentier M., Denys A, & Allain F. (2018). The Pro-Tumoral Activity of Heparan Sulfate 3-O-Sulfotransferase 3B (HS3ST3B) in Breast Cancer MDA-MB-231 Cells Is Dependent on the Expression of Neuropilin-1. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(10), 2718.

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Antibody Characterization for Gene Studies

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The antibody to Gene 33 has been described previously (Xu et al., 2005 (link); Xu et al., 2006 (link)). The antibody to γH2AX was purchased from Biolegend. The antibody to GAPDH was from Santa Cruz Biotechnology. Antibodies to UCHL1 and H2AX were obtained from Cell Signaling Technology. The antibody for KRBOX1 was purchased from Sigma-Aldrich. The siRNA oligo targeting KRBOX1 was obtained from Dharmacon.

Park S., Zhang X., Li C., Yin C., Li J., Fallon J.T., Huang W, & Xu D. (2017). Single-cell RNA Sequencing Reveals an Altered Gene Expression Pattern as a Result of CRISPR/cas9-mediated Deletion of Gene 33/Mig6 and Chronic Exposure to Hexavalent Chromium in Human Lung Epithelial Cells. Toxicology and applied pharmacology, 330, 30-39.

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Western Blot Antibodies for Signaling

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Antibodies to Akt (#9272, 1:1,000), phospho-Akt(S473) (#4060, 1:500), phospho-Akt(T308) (#13038, 1:500), p70 S6K (#9202, 1:1,000), phospho-P70 S6K(T389) (#9205, 1:500), 4E-BP1 (#39452, 1:1,000), phospho-4E-BP1(T37/46) (#9459, 1:500), S6 ribosomal protein (#2217, 1:1,000), phospho-S6 ribosomal protein(S240/244) (#2214, 1:1,000), LAMP1 (#3243, 1:1,000), Histone 3 (#4469, 1:1,000), Phospho-(Ser) 14-3-3 Binding Motif (#9601S, 1:500), Rictor (#2114, 1:500), Raptor (#2280, 1:500), ERK1/2 (#9102, 1:1,000), phosphor-ERK1/2 (#9101, 1:1,000), GSK-3β (D5C5Z) XP (#12456S, 1:1,000), phospho-GSK-3β (Ser9) (#9336S, 1:500), GFP (D5.1) (#29556, 1:1,000) and human TFEB (#4240, 1:500) were purchased from Cell Signaling. Antibody to GAPDH (#32233, 1:1,000) was purchased from Santa Cruz. Antibody to glial fibrillary acidic protein (GFAP) was purchased from DAKO (#Z0334, 1:1,000). Antibody to CD68 was purchased from AbD Serotec (#MCA1957, 1:1,000). Antibody to mouse TFEB was purchased from Proteintech (#13372-1-AP, 1:500). Mouse anti-FLAG M2 (#F1804, 1:1,000) and rabbit anti-FLAG (#F7425, 1:1,000) antibodies were purchased from Sigma. Pan 14-3-3 antibody (K-19) (#SC629, 1:300) was purchased from Santa Cruz. Antibody to TSC2 was purchased from Abcam (#32554, 1:1,000).

Palmieri M., Pal R., Nelvagal H.R., Lotfi P., Stinnett G.R., Seymour M.L., Chaudhury A., Bajaj L., Bondar V.V., Bremner L., Saleem U., Tse D.Y., Sanagasetti D., Wu S.M., Neilson J.R., Pereira F.A., Pautler R.G., Rodney G.G., Cooper J.D, & Sardiello M. (2017). mTORC1-independent TFEB activation via Akt inhibition promotes cellular clearance in neurodegenerative storage diseases. Nature Communications, 8, 14338.

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Quantification of MUC1 and MUC16 Proteins

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MUC1 and -16 proteins in cell lysates or present on the cell surface (isolated by capture of biotinylated cell surface proteins using the Pierce Pinpoint Cell Surface Protein Isolation Kit (ThermoScientific) following the manufacturer’s recommendations) [18] (link), [19] (link) were separated on 1% SDS-Agarose gels [20] (link), [21] , transferred to nitrocellulose and assayed by Western blot [22] (link), using antibodies 214D4 (Upstate) to MUC1 and anti-human CA125, Clone M11 (NeoMarkers) to MUC16. Blots were reprobed with antibody to GAPDH (Santa Cruz Biotechnology) as a loading control. Densitometric analyses of protein bands recognized by MUC1 or MUC16 antibodies were performed using 1D Image Analysis Software, Version 2.02 (Eastman Kodak, Co.). Data for total cellular MUC were expressed as MUC normalized to GAPDH and for cell surface MUC normalized per equivalent cm² of growth area and then both expressed relative to HCLE NT cells.

Gipson I.K., Spurr-Michaud S., Tisdale A, & Menon B.B. (2014). Comparison of the Transmembrane Mucins MUC1 and MUC16 in Epithelial Barrier Function. PLoS ONE, 9(6), e100393.

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Preparation of Cobalt Complexes and Antibodies

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Compounds 1 and 2, Co(bpy)Cl₂ were prepared as previously described
by the Boyd group.^{24 (link)} Co(phen)Cl₂ was prepared as described by Nami and Siddiqi.^{32 (link)} Antibodies to PARP, procaspase-8, cleaved caspase-8, procaspase-9,
cleaved caspase-9, Bim, Bcl-2, and BAD were obtained from Cell Signaling
Technology, Inc. (Danvers, MA). The antibody to GAPDH was obtained
from Santa Cruz Biotechnology (Dallas, TX).

Alghamdi N.J., Balaraman L., Emhoff K.A., Salem A.M., Wei R., Zhou A, & Boyd W.C. (2019). Cobalt(II) Diphenylazodioxide Complexes Induce Apoptosis in SK-HEP-1 Cells. ACS Omega, 4(11), 14503-14510.

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Cell Culture and Reagent Protocols

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Cell culture and reagents Huh-7, HepG2 and LO2 cells were obtained from the American Type Culture Collection. These cell lines were maintained in Dulbecco's Modi ed Eagle Medium (DMEM; GIBCO BRL) supplemented with 10% (v/v) FBS, 100 U/mL penicillin and 100 U/mL streptomycin. Cultures were maintained at 37℃ in a humidi ed at mosphere with 5% CO2. Sorafenib and Vertepor n were purchased from Selleck Chemicals.
Antibody to PARP, YAP, p-YAP(S127), survivin, Bcl-xl and Histone H3 were purchased from Cell Signaling Technology, Inc.; antibody to GAPDH was from Santa Cruz Biotechnology, Inc.; antibody to Flag was from Sigma.

Sun T., Mao W., Peng H., Wang Q, & Jiao L. (2021). YAP promotes sorafenib resistance in hepatocellular carcinoma by upregulating survivin. Cellular oncology (Dordrecht, Netherlands), 44(3).

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Cell Culture and Reagent Protocols

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Sun T., Mao W., Jiao L., Wan J., & Ming L. (2020). YAP promotes sorafenib resistance in hepatocellular carcinoma by upregulating survivin.

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