Prl tk renilla construct

Manufactured by Promega

Sourced in United States

The PRL-TK Renilla construct is a laboratory tool used for reporter gene assays. It serves as a control reporter to normalize transfection efficiency and cell viability in experiments involving dual-reporter systems.

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Lab products found in correlation

Dual luciferase kit, by Promega (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay kit, by Promega (1 mentions)

Close spelling variants of this product

PRL-TK Renilla luciferase construct PRL-TK construct PRL-TK Renilla PRL-TK Renilla construct Renilla-TK

2 protocols using prl tk renilla construct

Measuring FOXE1 Activity via Dual-Luciferase Assay

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FOXE1 activity was measured with the Dual-Luciferase Reporter Assay System (Promega).
250 ng of Luciferase reporter plasmid TG-Luc (27 (link)) were co-transfected with 80 ng of pRL-TK Renilla construct (Promega, Madison, Wisc., USA), 250 ng of FOXE1 constructs, and/or 250 ng of NKX2.1, PAX8 (27 (link)), empty vector as indicated in Figure 2 and Supplementary Figure 1. 24 hours post transfection luminescence was measured with Dual Luciferase kit (Promega) with Fluoroskan Ascent FL multiplate reader.

Grassi E.S., Rurale G., de Filippis T., Gentilini D., Carbone E., Coscia F., Uraghi S., Bullock M., Clifton-Bligh R.J., Gupta A.K, & Persani L. (2023). The length of FOXE1 polyalanine tract in congenital hypothyroidism: Evidence for a pathogenic role from familial, molecular and cohort studies. Frontiers in Endocrinology, 14, 1127312.

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Dual-Luciferase Reporter Assay Protocol

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HEK293T or ESCC cells with .1 μg of CLK4‐Luc promoter‐reporter or control reporter were co‐transfected with pRL‐TK Renilla construct (1 ng, Promega). Lipofectamine 2000 reagent (Invitrogen) was used to perform the dual‐luciferase reporter experiments. Two days later, the activity analysis of the luciferase reporter was monitored by using the Dual‐Luciferase Reporter Assay Kit (Promega).

Shen Y., Zhang H., Yao S., Su F., Wang H., Yin J., Fang Y., Tan L., Zhang K., Fan X., Zhong M., Zhou Q., He J, & Zhang Z. (2022). Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy. Clinical and Translational Medicine, 12(1), e719.

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