Rabbit anti eea1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-EEA1 is a primary antibody that recognizes the Early Endosome Antigen 1 (EEA1) protein. EEA1 is a key component of the early endosome and plays a role in endocytic membrane fusion. The anti-EEA1 antibody can be used to detect and localize EEA1 in various cellular and tissue samples.

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Lab products found in correlation

Lapatinib, by Selleck Chemicals (1 mentions) Chloroquine, by Merck Group (1 mentions) Dynasore, by Merck Group (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Dyngo 4a, by Selleck Chemicals (1 mentions) Anti phospho akt ser473 antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 labeled and, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 labeled secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti lamp2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti phospho mek1 2 ser217 221, by Cell Signaling Technology (1 mentions) Mouse anti β actin antibody, by Proteintech (1 mentions) 17 aag, by Cell Signaling Technology (1 mentions) Mouse anti α tubulin antibody, by Merck Group (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Puromycin, by Merck Group (1 mentions) Mouse anti ubiquitin p4g7 antibody, by Fortrea (1 mentions) Mouse anti gapdh, by Proteintech (1 mentions) Rabbit anti cox 4, by Cell Signaling Technology (1 mentions) Rabbit anti gm130, by Abcam (1 mentions) Mouse anti lamp1, by Santa Cruz Biotechnology (1 mentions)

3 protocols using rabbit anti eea1

Antibodies and Inhibitors for EGFR Signaling

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Mouse anti-EGFR (R1), mouse anti-RTN3, mouse anti-LAMP2, rabbit anti-EEA1, and rabbit anti-EGFR (1005) antibodies were purchased from Santa Cruz. Mouse anti-Ubiquitin (P4G7) antibody was obtained from Covance. Rabbit anti-phospho-MEK1/2 (Ser217/221) and anti-phospho-AKT (Ser473) antibodies were obtained from Cell Signaling Technology. Mouse anti-GAPDH and mouse anti-β-Actin antibodies were purchased from Proteintech (Wuhan, China). Mouse anti-α-Tubulin antibody was obtained from Sigma. Goat anti-rabbit and anti-mouse IRDye secondary antibodies (infrared-labeled) were purchased from LICOR. Alexa Fluor 488-labeled and Alexa Fluor 594-labeled secondary antibodies were obtained from Invitrogen. Gefitinib, lapatinib, filipin, and dyngo-4a were purchased from Selleck. EGF was purchased from PeproTech (USA). Cycloheximide was obtained from MP Biologicals. 17-AAG was purchased from Cell Signaling Technology. Chloroquine, puromycin, and dynasore were obtained from Sigma.

Wang T., Zhang J., Wang S., Sun X., Wang D., Gao Y., Zhang Y., Xu L., Wu Y., Wu Y., Liu F., Liu X., Liu S., Zhang Y., Wang Y., Zou L, & Liu H. (2018). The exon 19-deleted EGFR undergoes ubiquitylation-mediated endocytic degradation via dynamin activity-dependent and -independent mechanisms. Cell Communication and Signaling : CCS, 16, 40.

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Protein Localization and Modifications

Cited 1 time

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NAA80-EGFP and NAA80mut-EGFP were described previously [1 (link)]. mNeonGreen-ManII-N-10 was obtained from Allele Biotechnology, San Diego, CA, USA.
Primary antibodies used were mouse anti-Nt-acetylated β-Actin (Abcam, ab6276, Nt-Ac specificity validated in [1 (link)]), mouse anti-Actin-pan (Abcam, ab8224), rabbit anti-COXIV (Cell signalling Technology, 4850), rabbit anti-EEA1 (Santa Cruz Biotechnology, sc-33585), rabbit anti-GAPDH (Santa Cruz Biotechnology, sc-25778), rabbit anti-GM130 (Abcam, ab52649), mouse anti-GM130 (BD Biosciences, 610822), mouse anti-LAMP1 (Santa Cruz Biotechnology, sc-18821), rabbit anti-Mannosidase II (kind gift from Kelley Moremen University of Georgia, USA), rabbit anti-NAA80 (custom made, Biogenes GmbH, Berlin, Germany), mouse-PDI (Thermo Fisher Scientific Inc., MA3-018), sheep anti-TGN46 (Bio-Rad Laboratories Inc., AHP5006).

Beigl T.B., Hellesvik M., Saraste J., Arnesen T, & Aksnes H. (2020). N-terminal acetylation of actin by NAA80 is essential for structural integrity of the Golgi apparatus. Experimental cell research, 390(2), 111961.

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Immunofluorescence Analysis of SARS-CoV-2 Spike Protein

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The rabbit polyclonal anti-SARS spike antisera was a kind gift of Jim Wilson (University of Pennsylvania, PA). The rabbit polyclonal anti-BCV S antibody that recognizes HCoV-OC43-S protein was a kind gift from Brenda Hogue (Arizona state university). Rabbit anticalnexin was purchased from Stress gene (San Diego, CA), rabbit anti-β-COP from Affinity Bioreagents (Golden, CO), rabbit anti-MannII (Chemicon), rabbit anti-EEA1 and rabbit anti-Lamp1 from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Fluorescent-tagged secondary antibodies were obtained from Jackson Immunoresearch (West Grove, PA), Triton X-100 from Roche Diagnostics and tissue culture reagents form Invitrogen. Unless otherwise specified, all other reagents were from Sigma.

Sadasivan J., Singh M, & Sarma J.D. (2017). Cytoplasmic tail of coronavirus spike protein has intracellular targeting signals. Journal of Biosciences, 42(2), 231-244.

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