H1299 cells were transfected according to the figure panels for 48 hours followed by media harvest. 786-O and RCC4 cells were cultured in 21% or 1% O2 for 48 hours followed by media harvest. Growth factor reduced matrigel (Corning) was plated in a 96-well plate and allowed to set according to the manufacturer’s guidelines. GFP+ Human Umbilical Vein Endothelial Cells were plated on top of the matrigel in 10% EBM-2/EGM-2 (Lonza) and 90% conditioned media.
Ebm 2 egm 2
Manufactured by Lonza
The EBM-2/EGM-2 is a laboratory equipment product. It serves as a core function, but no further details can be provided in an unbiased and factual manner without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ebm 2 egm 2
1
Angiogenesis Assay with Conditioned Media
2
Angiogenesis Assay with Conditioned Media
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