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Goat anti brachyury af2085

Manufactured by R&D Systems
Sourced in United Kingdom

Goat anti-Brachyury (AF2085) is a primary antibody used for the detection of Brachyury, a transcription factor involved in mesoderm formation and notochord development. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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2 protocols using goat anti brachyury af2085

1

Embryonic Protein Expression Analysis

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Immunofluorescence was performed on frozen tissue sections of E8.5
embryos after prior methods (Wang and Matise,
2013
). Antibodies used were: goat anti-Brachyury (AF2085; R&D
Systems), goat anti-FOXA2 (M-20, sc-6554; Santa Cruz), goat anti-SOX1 (AF3369;
R&D Systems), rabbit anti-Ki67 (90584; Thermo), rabbit anti-phospho-Histone
H3 (06–570; Millipore), and rabbit anti-cleaved Caspase 3 (9661; Cell
Signaling). Immunofluorescence imaging was performed on a Zeiss Axio Imager
Z1.
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2

Immunohistochemical Antibody Panel

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Used in this study were mouse anti-brachyury (1H9A2, Abcam, UK), goat anti-brachyury (AF2085, R&D systems, USA), rabbit anti-TRAP (PAA902Mu01, Cloud-Clone Corp, USA), rabbit anti-AKT (#9272, Cell Signaling Technology (CST), USA), mouse Venus anti-phospho-AKT (#4051, CST), goat anti-cathepsin K (sc-6506, Santa Cruz Biotechnology, USA), rabbit anti-cathepsin B (#31,718, CST), and rabbit anti-RANK (H-300, Santa Cruz).
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