Goat anti brachyury af2085

Manufactured by R&D Systems

Sourced in United Kingdom

Goat anti-Brachyury (AF2085) is a primary antibody used for the detection of Brachyury, a transcription factor involved in mesoderm formation and notochord development. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Rabbit anti ki67, by Thermo Fisher Scientific (1 mentions) Goat anti sox1, by R&D Systems (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Rabbit anti phospho histone h3, by Merck Group (1 mentions) Axio imager z1, by Zeiss (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions)

2 protocols using goat anti brachyury af2085

Embryonic Protein Expression Analysis

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Immunofluorescence was performed on frozen tissue sections of E8.5
embryos after prior methods (Wang and Matise,
2013). Antibodies used were: goat anti-Brachyury (AF2085; R&D
Systems), goat anti-FOXA2 (M-20, sc-6554; Santa Cruz), goat anti-SOX1 (AF3369;
R&D Systems), rabbit anti-Ki67 (90584; Thermo), rabbit anti-phospho-Histone
H3 (06–570; Millipore), and rabbit anti-cleaved Caspase 3 (9661; Cell
Signaling). Immunofluorescence imaging was performed on a Zeiss Axio Imager
Z1.

Lewis E.M., Sankar S., Tong C., Patterson E., Waller L.E., Gontarz P., Zhang B., Ornitz D.M, & Kroll K.L. (2020). Geminin is required for Hox gene regulation to pattern the developing limb. Developmental biology, 464(1), 11-23.

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Immunohistochemical Antibody Panel

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Used in this study were mouse anti-brachyury (1H9A2, Abcam, UK), goat anti-brachyury (AF2085, R&D systems, USA), rabbit anti-TRAP (PAA902Mu01, Cloud-Clone Corp, USA), rabbit anti-AKT (#9272, Cell Signaling Technology (CST), USA), mouse Venus anti-phospho-AKT (#4051, CST), goat anti-cathepsin K (sc-6506, Santa Cruz Biotechnology, USA), rabbit anti-cathepsin B (#31,718, CST), and rabbit anti-RANK (H-300, Santa Cruz).

Kawaai K., Oishi Y., Kuroda Y., Tamura R., Toda M, & Matsuo K. (2024). Chordoma cells possess bone-dissolving activity at the bone invasion front. Cellular oncology (Dordrecht, Netherlands).

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