Graphite was purchased from AVONCHEM; glucosamine (GLA) was purchased from Merck; 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride (EDC) was purchased from Ambeed; triethyl amine (Et3N), sulfuric acid (H2SO4- 98%), and hydrochloric acid (HCl) were obtained from BDH laboratories; KMnO4 was used of analytical grade; hydrogen peroxide H2O2 (35%) was purchased from Scharlau chemicals; phosphoric acid, ethanol, and diethyl ether were purchased from Sigma Aldrich (St. Louis, MO, USA); and ethacridine lactate (EL) and sulfamethoxazole (Sul) were kind gifts from the local pharma. All these compounds were prepared and their working concentrations were used in different assays.
Ethacridine lactate
Manufactured by Merck Group
Sourced in Germany
Ethacridine lactate is a laboratory chemical compound used in various scientific and research applications. It is a pale yellow crystalline powder with a specific chemical formula and structure. Ethacridine lactate serves as a key component in various experimental and analytical procedures, though its precise core function may vary depending on the specific research context.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Chempur (3 mentions)
Uv 1280 spectrophotometer, by Shimadzu (3 mentions)
Calcium chloride, by Chempur (2 mentions)
Sulfamethoxazole, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
Gallotannin, by Merck Group (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated goat anti rabbit, by GE Healthcare (1 mentions)
Ibrutinib, by Selleck Chemicals (1 mentions)
Mitosox red, by Thermo Fisher Scientific (1 mentions)
Alamar blue, by Thermo Fisher Scientific (1 mentions)
Olaparib, by Selleck Chemicals (1 mentions)
Sulforhodamine b, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Puromycin, by Merck Group (1 mentions)
Z vad fmk, by Enzo Life Sciences (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Disodium hydrogen phosphate, by Merck Group (1 mentions)
5 protocols using ethacridine lactate
1
Synthesis and Characterization of Graphite-based Conjugates
2
Comprehensive Reagent Procurement for Signaling Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BTK and GAPDH antibodies were purchased from Cell Signaling Technology (Danvers, MA), BTK pTyr-223 antibody was obtained from Abcam (Cambridge, MA), and β-actin antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). HRP-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies were acquired from GE Healthcare (Buckinghamshire, UK). Kinase inhibitors ibrutinib, CC-292, ONO–4059, PIM1/2, and STO-609 were provided by the Ontario Institute for Cancer Research (Toronto, ON, Canada). ibrutinib was also obtained from Selleckchem (Houston, TX), as was olaparib. Z-VAD-FMK was purchased from Enzo Life Sciences (Farmingdale, NY). Ethacridine lactate, gallotannin, sulforhodamine-B, hydrogen peroxide, puromycin, and shRNA plasmid-containing bacterial glycerol stocks were purchased from Sigma-Aldrich (St. Louis, MO). The library of internationally prescribed drugs was purchased from MicroSource Discovery Systems, Inc. (Gaylordsville, CT). Alamar Blue was purchased from Life Technologies (Carlsbad, CA) and carboxy-H2DCFDA-FITC and MitoSOX Red were obtained from Molecular Probes/Life Technologies (Eugene, OR).
3
Ethacridine Lactate Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were of analytical grade and used as received.
Ethacridine lactate, potassium dihydrogen phosphate, and disodium hydrogen phosphate were obtained from Merck (Darmstadt, Germany). Hydrochloride acid, sodium chloride, and ethanol were purchased from Chemical Company (Iași, Romania). Sodium hydroxide and calcium chloride were delivered by Chempur (Piekary Ślaskie, Poland). Sodium alginate (low viscosity grade) was bought from BUCHI Laboratortechnik AG (Flawil, Switzerland).
All solutions were prepared with distilled water. When required, pH was adjusted with NaOH (0.1 M) or HCl (0.1 M).
A 500 mg/L stock solution of Ethacridine lactate was prepared and set aside at 4 °C in a closed vessel. Dilutions with concentrations between 1 and 60 mg/L were then obtained; their absorbance, recorded at a wavelength of 431 nm on a UV1280 spectrophotometer (Shimadzu, Tokyo, Japan), served for the construction of a calibration curve.
All the experiments were carried out in triplicate.
Ethacridine lactate, potassium dihydrogen phosphate, and disodium hydrogen phosphate were obtained from Merck (Darmstadt, Germany). Hydrochloride acid, sodium chloride, and ethanol were purchased from Chemical Company (Iași, Romania). Sodium hydroxide and calcium chloride were delivered by Chempur (Piekary Ślaskie, Poland). Sodium alginate (low viscosity grade) was bought from BUCHI Laboratortechnik AG (Flawil, Switzerland).
All solutions were prepared with distilled water. When required, pH was adjusted with NaOH (0.1 M) or HCl (0.1 M).
A 500 mg/L stock solution of Ethacridine lactate was prepared and set aside at 4 °C in a closed vessel. Dilutions with concentrations between 1 and 60 mg/L were then obtained; their absorbance, recorded at a wavelength of 431 nm on a UV1280 spectrophotometer (Shimadzu, Tokyo, Japan), served for the construction of a calibration curve.
All the experiments were carried out in triplicate.
4
Ethacridine Lactate Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents necessary for the present research work were of analytical purity and were used without prior treatment or purification.
Ethacridine lactate (Figure 1 ) was bought from Merck (Darmstadt, Germany). Hydrochloride acid, sodium chloride and ethanol were provided by Chemical Company (Iași, Romania). Sodium hydroxide and calcium chloride were purchased from Chempur (Piekary Ślaskie, Poland). Sodium alginate (low viscosity grade) was procured from BUCHI Laboratortechnik AG (Flawil, Switzerland).
Residual biomass of Saccharomyces pastorianus was kindly donated by Albrau Company (Onești, Romania).
All the solutions were prepared in distilled water. When needed, NaOH (0.1 M) or HCl (0.1 M) were used to adjust the pH.
From a 500 mg/L stock solution of Ethacridine lactate, kept at 4 °C in a closed vessel, dilutions within concentration range from 1 mg/L to 70 mg/L were obtained. Their absorbance was read at 431 nm on a UV1280 spectrophotometer (Shimadzu, Tokyo, Japan) and served for plotting the calibration curve.
All the experiments were carried out in triplicate.
Ethacridine lactate (
Residual biomass of Saccharomyces pastorianus was kindly donated by Albrau Company (Onești, Romania).
All the solutions were prepared in distilled water. When needed, NaOH (0.1 M) or HCl (0.1 M) were used to adjust the pH.
From a 500 mg/L stock solution of Ethacridine lactate, kept at 4 °C in a closed vessel, dilutions within concentration range from 1 mg/L to 70 mg/L were obtained. Their absorbance was read at 431 nm on a UV1280 spectrophotometer (Shimadzu, Tokyo, Japan) and served for plotting the calibration curve.
All the experiments were carried out in triplicate.
5
Ethacridine Lactate Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents required for conducting the experiments were of analytical quality and did not undergo any treatment or purification.
Ethacridine lactate was purchased from Merck (Darmstadt, Germany). Hydrochloride acid, sodium chloride, and ethanol were delivered by Chemical Company (Iași, Romania). Sodium hydroxide and calcium chloride were bought from Chempur (Piekary Ślaskie, Poland). Sodium alginate (low viscosity grade) was procured from BUCHI Laboratortechnik AG (Flawil, Switzerland).
Saccharomyces pastorianus, in the form of dried and residual biomass, were a gentle donation of the brewing company Albrau (Onești, Romania).
Distilled water was used to prepare all the solutions. NaOH (0.1 M) or HCl (0.1 M) were employed in pH corrections.
A stock solution of Ethacridine lactate with a concentration of 500 mg/L was firstly prepared and kept at 4 °C in a closed vessel. Subsequent dilutions (1 mg/L to 60 mg/L) were obtained; their absorbance was recorded at a wavelength of 431 nm on a UV1280 spectrophotometer (Shimadzu, Tokyo, Japan) and served to plot the calibration curve.
All the experiments were performed in triplicate.
Ethacridine lactate was purchased from Merck (Darmstadt, Germany). Hydrochloride acid, sodium chloride, and ethanol were delivered by Chemical Company (Iași, Romania). Sodium hydroxide and calcium chloride were bought from Chempur (Piekary Ślaskie, Poland). Sodium alginate (low viscosity grade) was procured from BUCHI Laboratortechnik AG (Flawil, Switzerland).
Saccharomyces pastorianus, in the form of dried and residual biomass, were a gentle donation of the brewing company Albrau (Onești, Romania).
Distilled water was used to prepare all the solutions. NaOH (0.1 M) or HCl (0.1 M) were employed in pH corrections.
A stock solution of Ethacridine lactate with a concentration of 500 mg/L was firstly prepared and kept at 4 °C in a closed vessel. Subsequent dilutions (1 mg/L to 60 mg/L) were obtained; their absorbance was recorded at a wavelength of 431 nm on a UV1280 spectrophotometer (Shimadzu, Tokyo, Japan) and served to plot the calibration curve.
All the experiments were performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!