NKp44-Ig, NKp46-Ig, Ncr1-Ig, NKp30-Ig, KIR2DL1-Ig and KIR2DS4-Ig (2μg) were run on 10% SDS-PAGE gels in reducing conditions and blotted with 0.4μg/ml of biotinylated HA-Ig or with biotinylated anti-human IgG (0.1μg/ml) and then incubated with Avidin-HRP (Bio Legend). For NA treatment, NKp44-Ig and NKp46-Ig fusion protein were incubated with NA beads (Sigma) at a ratio of 3.5μl NA beads for 1μg fusion protein and were treated or not with 25ul oseltamivir carboxylate (10ug-10mg/ml). Samples (4μg) were run on 10% SDS-PAGE gels and blotted with 20 μg/ml of biotinylated SNA lectin (Vector laboratories) or with 0.4μg/ml of biotinylated HA-Ig and then incubated for 30 minutes with Avidin-HRP (Bio Legend).
Na beads
Manufactured by Merck Group
NA beads are a type of resin-based chromatography media used for the purification of nucleic acids. They are composed of an agarose-based matrix that binds and captures nucleic acid molecules, allowing for their separation and isolation from complex biological samples.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using na beads
1
Lectin-Binding Profiles of NK Cell Receptors
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