The largest database of trusted experimental protocols

Adenosine

Manufactured by Tokyo Chemical Industry
Sourced in Japan, China

Adenosine is a naturally occurring nucleoside found in all living cells. It is a core component of adenosine triphosphate (ATP), the primary energy currency of the cell. Adenosine plays a vital role in various cellular processes, including energy production, signal transduction, and nucleic acid synthesis.

Automatically generated - may contain errors

3 protocols using adenosine

1

Cell Proliferation Assay of Cordyceps militaris

Check if the same lab product or an alternative is used in the 5 most similar protocols
3-(4,5-dimethylthiazol-2-yi)-2,5-diphenyltetrazolium bromide (MTT) from Sigma-Aldrich (Burlington, MA, USA) was used for measuring cell proliferation potential. Cordyceps militaris fruiting bodies were produced in South Korea (Mushtech Co., Ltd., Gangwon, Republic of Korea), dried at 45 °C, 55 °C, and 65 °C in stages and frozen at −25 °C. Sweet potato shochu was produced by Kirishima Shuzo Co., Ltd. (Miyazaki, Japan); Adenosine (>98.0%) was purchased from Tokyo Chemical Industries (TCI, Tokyo, Japan). 3′-DeoxyAdenosine (Cordycepin (>98.0%)) was produced by Fujifilm Wako Chemicals (Osaka, Japan). N6-(2-hydroxyethyl)-Adenosine (>99.0%) was purchased from GlpBio Technology (Montclair, CA, USA). 3-propyl-6-ethyl-5-[(ethylthio)carbonyl]-2 phenyl-4-propyl-3-pyridine carboxylate (MRS1523, Adenosine A3 receptor antagonist) was purchased from Sigma Chemical (St. Louis, MO, USA). Rabbit Polyclonal Adenosine A3 Receptor antibody (GTX131656) was purchased from Gene Tex (Los Angeles, CA, USA).
+ Open protocol
+ Expand
2

Quantitative Analysis of Epimedin C

Check if the same lab product or an alternative is used in the 5 most similar protocols
Epimedin C (purity ≥ 98%), used as the dosage administration, was purchased from Cdmust Biology Technology Ltd. (Chengdu, China). Lamivudine (internal standard, IS, purity > 98%), adenosine (purity > 99%), guanosine (purity > 98%), and cytidine (purity > 98%) were purchased from Tokyo Chemical Industry (Shanghai) (Shanghai, China). Uridine (purity 99%), m6A (purity 97%), and m5C (purity 98%) were purchased from J&K Chemical (Shanghai, China). nuclease P1 and alkaline phosphatase were purchased from Takara Biotechnology (Dalian, China). Methanol (HPLC grade) and Formic acid (chromatographic grade) were obtained from Fisher Scienti c (Shanghai, China). Ultra-puri ed water was used throughout this study and was prepared using a Milli-Q puri cation system (Millipore, Milford, MA, USA). All of the other chemicals and reagents were of analytical grade.
+ Open protocol
+ Expand
3

Adenosine and 15N5-adenosine Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adenosine and 15 N5-Adenosine were purchased from Tokyo Chemical Industry Co., Ltd. and Silantes, respectively, and used as received without further purification. The LC-MS grades of acetonitrile and ammonium acetate were available from Kanto Chemical Co., Inc. and Fluka, respectively. Pooled human plasma was purchased from Kohjin Bio (Cat. Code: 12250110).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!