Bv510 anti mouse cd45 antibody

Manufactured by BioLegend

Sourced in United States

The BV510 anti-mouse CD45 antibody is a flow cytometry reagent that specifically binds to the CD45 surface antigen expressed on mouse immune cells. It is designed to facilitate the identification and analysis of various leukocyte populations in mouse samples.

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Anti-CD45 (228 citations) CD45-BV510 (39 citations) Anti-mouse CD45 (39 citations) Anti-CD45 antibody (28 citations) Anti-CD45 BV510 (10 citations) Anti-mouse cd45-bv510 (5 citations) Anti-mouse CD45 antibody (4 citations) BV510-CD45 antibody (2 citations)

2 protocols using bv510 anti mouse cd45 antibody

Comprehensive Flow Cytometry Antibody Panel

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The following flow cytometry antibodies were purchased from BioLegend company (San Diego, USA): CD16/32 (clone: 93), BV510 anti-mouse CD45 antibody (clone: 30-F11), BV711 anti-mouse CD8a antibody (clone: 53-6.7), APC anti-mouse CD3 antibody (clone: 17A2), BV421 anti-mouse Foxp3 antibody (clone: MF-14), PE anti-mouse CD206 antibody (clone: C068C2), BV605 anti-mouse CD11b antibody (clone: M1/70), PE/Cy7 anti-mouse F4/80 antibody (clone: QA17A29), BV650 anti-mouse CD25 antibody (clone: PC61), Percp/Cy5.5 anti-mouse Gr-1 (Ly-6G/Ly-6C) antibody (clone: RB6-8C5), APC/Cy7 anti-mouse CD86 antibody (clone: GL-1), FITC anti-mouse CD80 antibody (clone: 16-10A1), PE/Cy5 anti-mouse CD19 (clone: 6D5), and Alexa fluor 700 anti-mouse MHC class II antibody (clone: M5/114.15.2). Flow cytometry antibodies for BUV563 anti-mouse CD4 (clone: RM4-5) was purchased from BD Company (New York, USA).

Cao Z., Li D., Zhao L., Liu M., Ma P., Luo Y, & Yang X. (2022). Bioorthogonal in situ assembly of nanomedicines as drug depots for extracellular drug delivery. Nature Communications, 13, 2038.

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Multicolor Flow Cytometry Immunophenotyping

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Cells were incubated with Fc block antibody (#14-0161-82, eBioscience, 1:100) for 30 min at 4 °C to prevent nonspecific binding. Cell surface labeling was conducted with fluorescently conjugated antibodies for 30 min at 4°C. The following antibodies were used: BV510 anti-mouse CD45 antibody (#103137, Biolegend, 1:25), PerCP anti-mouse CD11b (#101229, Biolegend, 1:50), APC anti-mouse F4/80 (#17-4801-80, eBioscience, 1:25), PE/Cy7 anti-mouse Gr1 (#108415, Biolegend, 1:100), FITC anti-mouse CD3 (#11-0032-82, eBioscience, 1:50), PE/Cy7 anti-mouse CD19 (#25-0193-81, eBioscience, 1:50), BV421 anti-mouse NK1.1 (#108741, Biolegend, 1:20), PE anti-mouse CD36 (#562702, BD Biosciences, 1:100), PE anti-mouse CD4 (#4329629, Invitrogen, 1:200), APC anti-mouse CD8a (#17-0081-81, eBioscience, 1:200), BV421 anti-mouse CD206 (#141717, Biolegend, 1:25), FITC anti-mouse CD80 (#FITC-65076, Proteintech, 1:200), FITC anti-mouse GzmB (#372206, Biolegend, 1:25), FE anti-mouse IFNγ (#PE-65153, Proteintech, 1:50). Fluorescence data were collected using a FACSAria II flow cytometer (BD Biosciences, USA) and analyzed employing a FlowJo software. The single cell population was separated with a BD FACSAria II Cell Sorter.

Yang P., Qin H., Li Y., Xiao A., Zheng E., Zeng H., Su C., Luo X., Lu Q., Liao M., Zhao L., Wei L., Varghese Z., Moorhead J.F., Chen Y, & Ruan X.Z. (2022). CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis. Nature Communications, 13, 5782.

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