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2 protocols using anti p akt thr308 ab

1

Antibody Acquisition for Cell Signaling

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Anti‐GAPDH Ab was purchased from Santa Cruz Biotechnology. Anti‐Akt Ab, anti‐p‐Akt (Thr308) Ab, anti‐PI3‐kinase p85 Ab, anti‐mouse‐HRP secondary Ab, anti‐His antibody anti‐SLC1A5 antibody, anti‐goat‐HRP secondary Ab, anti‐ATF4 antibody, and anti‐rabbit‐HRP secondary Ab were purchased from Cell Signaling Technology. Anti‐COL1A1 antibody and anti‐alpha‐SMA antibody were purchased from Abcam. Biotinylated Concanavalin A (Con A) and Biotinylated PHA‐L (Phaseolus Vulgaris Leucoagglutinin) were purchased from Vector Laboratories.
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2

Western Blot Analysis of Key Proteins

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Western blot analysis was performed as previously described.[9
] Primary antibodies included: mouse monoclonal anti‐CD133 (W6B3C1 clone) (Miltenyi Biotec), rabbit monoclonal anti‐GAPDH Ab (Cell signaling), anti‐Akt Ab (Cell signaling), anti‐p‐Akt (Thr308) Ab (Cell signaling), anti‐PI3‐kinase p85 Ab (Millipore), and anti‐SLC1A5 antibody (Cell signaling.
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