HDFs and CAFs were treated with different inhibitors or agonists in complete DMEM media containing 10% FBS and 1% antibiotics for a duration of 48-72 hours as specified in figure legends. HDFs were treated with the AR inhibitor AZD3514 (10 μM, Adooq Biosciences, Cat# A12396), the PPP1 inhibitor Tautomycin (10 μM, SantaCruz, Cat# sc-507214) or DMSO as solvent control. CAFs were treated with the AR agonist Ostarine (Selleckchem, Cat# S1174) or DMSO. Furthermore, HDFs were cultured in DMEM supplemented with charcoal-treated FBS for 48 hours prior to treatment with DHT (10 nM, Sigma-Aldrich, Cat# 10300), the AR degrading PROTAC ARCC4 (1 μM, Tocris, Cat# 7254), or ethanol, and treatment was continued for the specified duration.
Tautomycin
Manufactured by Santa Cruz Biotechnology
Sourced in Canada
Tautomycin is a potent inhibitor of protein phosphatases. It functions by selectively targeting and inhibiting the catalytic activity of protein phosphatases, which are enzymes responsible for the dephosphorylation of proteins. This inhibition can have significant effects on cellular signaling pathways and various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Ostarine, by Selleck Chemicals (1 mentions)
Donkey anti goat igg hrp, by Santa Cruz Biotechnology (1 mentions)
Gsk650394, by Bio-Techne (1 mentions)
Fostriecin, by Bio-Techne (1 mentions)
Anti flag m2 peroxidase, by Merck Group (1 mentions)
Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions)
Sanguinarine chloride, by Bio-Techne (1 mentions)
Normal mouse igg, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
Complete protease inhibitor cocktail tablet, by Roche (1 mentions)
Okadaic acid, by Merck Group (1 mentions)
2 protocols using tautomycin
1
Modulation of Fibroblast Signaling
2
Investigating Regulatory Protein Interactions
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Simvastatin, rifampicin, pravastatin sodium salt hydrate, phosphatase inhibitor cocktail 2, phosphatase inhibitor cocktail 3, ANTI-FLAG M2 Affinity Gel, ANTI-FLAG M2-Peroxidase (HRP) and monoclonal ANTI-FLAG M1 antibodies were purchased from Sigma-Aldrich (St. Louis, MO); restriction endonucleases and DNA-modifying enzymes from New England Biolabs (Beverly, MA); cOmplete Protease Inhibitor Cocktail Tablets (Roche, Basel, Switzerland); okadaic acid from EMD Millipore (Billerica, MA); fostriecin, sanguinarine chloride and GSK 650394 from Tocris Bioscience (Bristol, UK); anti-PXR antibody from Perseus Proteomics Inc. (Tokyo, Japan); anti-SGK2 antibody and anti-PP2Cα (D18C10) XP antibody from Cell Signaling (Danvers, MA); anti-PP2Ac antibody from BD Biosciences (Franklin Lakes, NJ); anti-p-SGK antibody (Thr256), anti-β-actin antibody (C4), PP1α (C-19) antibody, anti-RXR (ΔN 197) antibody. goat anti-rabbit IgG-HRP, goat anti-mouse IgG-HRP, donkey anti-goat IgG-HRP, normal mouse IgG and tautomycin from Santa Cruz Biotechnology (Santa Cruz, CA).
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