Fibrin matrices were prepared by mixing human fibrinogen (plasminogen-, von Willebrand Factor-, and fibronectin-depleted; 25 mg/mL; Enzymes Research Laboratories, Indiana, USA), fluorescent Alexa 647-conjugated fibrinogen (0.5 mg/mL; Invitrogen, California, USA), factor XIIIa (3 U/mL; CSL Behring, Pennsylvania, USA), and thrombin (6 U/mL; Sigma-Aldrich, Missouri, USA) with 2.5 mM Ca2+ in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Hepes) (Lonza, Basel, CH). Matrices containing TG-VEGF and TG-PDG-BB were obtained by adding the engineered proteins to the cross-linking enzymes solution before mixing with fibrinogen.
Alexa 647 conjugated fibrinogen
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 647-conjugated fibrinogen is a fluorescently-labeled version of the plasma protein fibrinogen. Fibrinogen is a key component in the coagulation cascade and is involved in the formation of blood clots. The Alexa Fluor 647 dye allows for the detection and tracking of fibrinogen in various research applications.
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2 protocols using alexa 647 conjugated fibrinogen
1
Engineered Fibrin Matrix Preparation
2
Engineered Fibrin Matrices with Growth Factors
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Fibrin matrices were prepared by mixing human fibrinogen (plasminogen-, von Willebrand Factor-, and fibronectin-depleted; 25 mg/mL; Enzymes Research Laboratories, IN, United States), fluorescent Alexa 647-conjugated fibrinogen (0.5 mg/mL; Invitrogen, CA, United States), factor XIIIa (3 U/mL; CSL Behring, PA, United States), and thrombin (6 U/mL; Sigma-Aldrich, MO, United States) with 2.5 mM Ca2+ in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Hepes) (Lonza, Basel, CH). Matrices containing TG-VEGF and TG-PDG-BB were obtained by adding the engineered proteins to the cross-linking enzymes solution before mixing with fibrinogen.
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