During experiments, tissues were maintained in Krebs’ solution consisting of (mM): NaCl 118.4, KCl 4.7, CaCl2 2.5, MgSO4 1.2, KH2PO4 1.2, NaHCO3 25 and glucose 11.7. ATP, tetraethylammonium chloride (TEA), tetrodotoxin, atropine sulfate monohydrate and carbachol were obtained from FUJIFILM-Wako (Osaka, Japan). D-tubocurarine, suramin, methoctramine hydrate, 4-diphenylacetoxy-N-methyl-piperidine methiodide (4-DAMP), pyridoxal phosphate-6-azophenyl-2,4-disulfonic acid ( PPADS), cibacron blue F3GA (CBF3GA; synonym reactive blue 2) and 4-aminopyridine (4-AP) were obtained from Sigma-Aldrich (St Louis, MO, USA). Glibenclamide and nicorandil were obtained from Tokyo Chemical Industry (Tokyo, Japan). GSK1016790A was obtained from Cayman Chemical (Ann Arbor, MI, USA). Apamin was obtained from Peptide Institute (Osaka, Japan). tetrodotoxin was dissolved in citrate solution. Glibenclamide, nicorandil and GSK1016790A were dissolved in DMSO. Other drugs were dissolved in distilled water. The highest concentration of vehicles (0.1%) for the drugs alone had no effect on the basal tone and contractile responses at the concentrations used. The concentrations of drugs given were final concentrations in the bath solution.
Methoctramine hydrate
Manufactured by Merck Group
Sourced in United States, Israel
Methoctramine hydrate is a chemical compound used as a laboratory reagent. It functions as a muscarinic acetylcholine receptor antagonist.
Automatically generated - may contain errors
Lab products found in correlation
Suramin, by Merck Group (1 mentions)
Carbachol, by Fujifilm (1 mentions)
Tetrodotoxin, by Fujifilm (1 mentions)
Apamin, by Peptide Institute (1 mentions)
Atropine sulfate monohydrate, by Fujifilm (1 mentions)
D tubocurarine, by Merck Group (1 mentions)
Gsk1016790a, by Cayman Chemical (1 mentions)
Pyridoxalphosphate 6 azophenyl 2 4 disulfonic acid ppads, by Merck Group (1 mentions)
4 diphenylacetoxy n methyl piperidine methiodide 4 damp, by Merck Group (1 mentions)
Cibacron blue f3ga, by Merck Group (1 mentions)
Glibenclamide, by Tokyo Chemical Industry (1 mentions)
Adenosine triphosphate (atp), by Fujifilm (1 mentions)
4 aminopyridine 4 ap, by Merck Group (1 mentions)
Retigabine, by MedChemExpress (1 mentions)
Xe 991 dihydrochloride, by MedChemExpress (1 mentions)
Ica 27243, by Alomone (1 mentions)
Chromanol 293b, by Bio-Techne (1 mentions)
Tropicamide, by Merck Group (1 mentions)
Trihexyphenidyl hydrochloride, by Merck Group (1 mentions)
Scopolamine hydrobromide, by Merck Group (1 mentions)
4 protocols using methoctramine hydrate
1
Krebs' Solution Preparation for Tissue Experiments
2
Investigating Ion Channel Modulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pentobarbital was obtained from The Norwegian National Hospital, Oslo, Norway; retigabine (ethyl-(2-amino-4-(4-fluorobenzylamino)-phenyl)carbamate dihydrochloride) and XE-991 dihydrochloride (10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone) from MedChem Express, Princeton, NJ, USA; chromanol 293B (trans-N-[6-Cyano-3,4-dihydro-3-hydroxy- -2,2-dimethyl-2H-1-benzopyran-4-yl]-N-methyl-ethanesulfonamide) from Tocris Bioscience, Bristol, UK; ICA-27243 (N-(6-chloro-pyridin-3-yl)-3,4-difluoro-benzamide) from Alomone Labs, Jerusalem, Israel; and methoctramine hydrate (N,N'-bis[6-[(2-methoxybenzyl)amino]hexyl]-1, 8-octanediamine tetrahydrochloride) from Sigma Chemical Co., St. Louis, MO, USA.
3
Pharmacological Modulation of Cognitive Function
4
Pharmacological Modulation of Neuronal Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(−)-Bicuculline (BIC) chloride (20 µM, an A-type γ-aminobutyric acid [GABA] receptor antagonist), D-(−)-2-amino-5-phosphonopentanoic acid (AP5, 10 µM, a N-methyl-D-aspartate receptor antagonist), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, AMPA/kainate receptor antagonist), tetrodotoxin citrate (TTX, 0.5 µM, a voltage-gated sodium channel blocker), acetylcholine (ACh, 100 µM, non-selective AChR agonist), scopolamine hydrobromide (10 nM, non-selective mAChR antagonist), mecamylamine hydrochloride (10 nM, non-selective nAChR antagonist), (−)-nicotine tartrate (100 µM, nAChR agonist), PNU282987 (10 µM, alpha7 nAChR agonist), TC2559 difumarate (10 µM, alpha4beta2 nAChR agonist), and CC4 (10 uM, alpha6beta2 nAChR agonist) were all purchased from Cayman Chemical (Ann Arbor, MI). Nifedipine (1 µM, L-type voltage-gated calcium channel blocker), (+)-muscarine chloride (Mus,10 µM, non-selective mAChR agonist), cesium chloride (Cs, 5 mM, broad-spectrum blocker of inwardly rectifying potassium (Kir) channels), methoctramine hydrate (50 nM, M2 mAChR antagonist) and phorbol 12-myristate 13-acetate (PMA, 50 nM, protein kinase C (PKC) activator) were purchased from Sigma-Aldrich. NS3861 (10 µM, alpha3beta2 and alpha3beta4 nAChR agonist) and PD102807 (400 nM, M4 mAChR antagonist) were purchased from Tocris (Bristol, United Kingdom).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!