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Anti human cd14

Manufactured by Abcam
Sourced in United States

Anti-human CD14 is a laboratory reagent used for the detection and analysis of CD14, a cell surface glycoprotein that serves as a co-receptor for the detection of bacterial lipopolysaccharide. This product provides a tool for researchers to study the expression and function of CD14 in various biological and clinical applications.

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2 protocols using anti human cd14

1

Characterization of Intestinal Immune Cells in CD

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Small intestinal tissues from patients with active-phase CD were obtained after ileocecal resection due to intractability to medical treatment. Briefly, tissue samples were fixed with 10% buffered formalin, embedded in paraffin, and stained with hematoxylin and eosin. For immunofluorescence analysis, paraffin sections were incubated with anti-human AIM (AnaSpec, Fremont, CA, USA), anti-human CD14 (Abcam plc, Cambridge, UK), and anti-human CD16 (AbD Serotec, Oxford, UK), followed by imaging under a fluorescence microscope.
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2

Western Blot Antibody Panel for Immune Response

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Primary antibodies for western blot were as follows: anti-MD2 (rabbit polyclonal, Abcam, 1:500), anti-TLR4 (rabbit polyclonal, Santa Cruz, 1:200), anti-GFAP (rat monoclonal, Invitrogen/Life Technologies, 1:1000), anti-Iba-1 (mouse monoclonal, Abcam, 1:1000), anti-iNOS (rabbit polyclonal, Novus Biologicals, 1:500), anti-human CD14 that is known to cross-react with mouse CD14 (rabbit polyclonal, Abcam, 1:200), anti-human CD14 (rabbit polyclonal, Thermo Scientific, 1:100), anti-human CD14 (rabbit polyclonal, Sigma, catalogue #HPA001887 at 1:250), anti-mouse CD14 (rat monoclonal, BD Biosciences, catalogue #553738 at 1:250), and anti-β-actin (mouse monoclonal, Cell Signaling, 1:500).
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