Cntnap4 knock-in mice used in this study were generated by introducing eGFP in frame with Cntnap4 start codon. To characterize gene expression, eGFP was used to represent Cntnap4-expressing cells in the brain. Western blot analysis for Cntnap4 localization was performed on adult mouse cortex using a Cntnap4 rabbit polyclonal antibody described previously8 . Cntnap4-Fc fusion protein was applied to live neuronal cultures for in vitro localization studies. Evoked extracellular dopamine release was measured using fast-scan cyclic voltammetry of P60 in vitro slice preparations in WT, HET and KO animals11 . For paired cell recordings between FS and excitatory neurons, Cntnap4 mice were crossed to a parvalbumin-cre:RFP reporter background to enable targeted physiological recordings. For synaptic ultrastructural analysis, P60 Cntnap4 WT, HET and KO animals were analyzed by electron microscopy, focusing on perisomatic inhibitory synaptic contacts. Cntnap4 mice were assessed by a number of behavioral analyses including grooming score and pre-pulse inhibition28 ,29 (San Diego Instruments). Pharmacological rescue was performed using indiplon (Tocris), which was administered acutely by oral gavage as well as haloperidol (Innovative Research of America), which was delivered chronically via subcutaneous slow-release pellet27 .
Haloperidol
Manufactured by Innovative Research
Haloperidol is a pharmaceutical compound used as a laboratory reagent. It is a synthetic drug that acts as an antipsychotic medication. The core function of Haloperidol is to serve as a tool for researchers in various scientific investigations and studies.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using haloperidol
1
Cntnap4 Knock-in Mouse Model: Neurobiological and Behavioral Insights
2
Haloperidol-Induced Latency Measurement in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were performed according to protocols approved by the Stanford Institutional Animal Care and Use Committee. All mice were obtained from Jackson Labs, and were used at 10–12 weeks of age, and the results are reported according to the ARRIVE guidelines (S1 Text ) [21 (link)]. Haloperidol was administered to the mice by one of two methods: (i) a Haloperidol pellet (Innovative Research of America) was subcutaneously implanted, which continuously released a dosage equivalent to 3 mg/kg/day, using published methods [22 (link)]; or (ii) with 10 mg/kg Haloperidol (Sigma) IP qd for measurement of drug and metabolite concentrations. The Haloperidol-induced latency was measured as the time required for a mouse to move all four paws after being placed on a vertical metal mesh screen as previously described [22 (link)].
3
Cntnap4 Knock-in Mouse Model: Neurobiological and Behavioral Insights
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cntnap4 knock-in mice used in this study were generated by introducing eGFP in frame with Cntnap4 start codon. To characterize gene expression, eGFP was used to represent Cntnap4-expressing cells in the brain. Western blot analysis for Cntnap4 localization was performed on adult mouse cortex using a Cntnap4 rabbit polyclonal antibody described previously8 . Cntnap4-Fc fusion protein was applied to live neuronal cultures for in vitro localization studies. Evoked extracellular dopamine release was measured using fast-scan cyclic voltammetry of P60 in vitro slice preparations in WT, HET and KO animals11 . For paired cell recordings between FS and excitatory neurons, Cntnap4 mice were crossed to a parvalbumin-cre:RFP reporter background to enable targeted physiological recordings. For synaptic ultrastructural analysis, P60 Cntnap4 WT, HET and KO animals were analyzed by electron microscopy, focusing on perisomatic inhibitory synaptic contacts. Cntnap4 mice were assessed by a number of behavioral analyses including grooming score and pre-pulse inhibition28 ,29 (San Diego Instruments). Pharmacological rescue was performed using indiplon (Tocris), which was administered acutely by oral gavage as well as haloperidol (Innovative Research of America), which was delivered chronically via subcutaneous slow-release pellet27 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!