Sources of various chemicals and reagents used in these studies are as follows: BAPTA (Calbiochem); BSA (Thermo Fisher); Dulbecco’s PBS (Gibco Life Technologies); bovine liver catalase (Sigma Aldrich); CCCP (Calbiochem); CellTiter 96® Aqueous One solution (Promega); FBS (Atlanta Biologicals); oligomycin (Sigma Aldrich); Matrigel® (BD Biosciences); TBB, TBCA and thapsigargin (EMD Millipore); trypsin + EDTA (Gibco Invitrogen); and JC-1 (Life Technologies). The following antibodies were used for western blot analysis: AKT-1 phospho-Ser129 (1:1000, Epitomics 5508-1); AKT-1 (1:1000, Cell Signaling 9272); actin (1:1000, Santa Cruz sc-1616); cleaved caspase-3 (1:1000, Cell Signaling 9661); caspase-9 (1:1000, Cell Signaling 9508); lamin A/C (1:1000, Cell Signaling 2032); CKII α (1:3000, Bethyl Laboratories A300-197); CKII α′ (1:3000, Bethyl Laboratories A300-199); cytochrome c (1:10,000, Epitomics 2119-1); Bax (1:1000, Cell Signaling 2772); Bid (1:1000, Cell Signaling 2002); and Cox IV (1:1000, Cell Signaling 4850).
Qaiser F., Trembley J.H., Kren B.T., Wu J.J., Naveed A.K, & Ahmed K. (2014). Protein kinase CK2 inhibition induces cell death via early impact on mitochondrial function*. Journal of cellular biochemistry, 115(12), 2103-2115.
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