The antibodies were used as following, NAT10 (13365-1-AP, Proteintech; DF12427, Affinity), CDK4 (11026-1-AP, Proteintech), CDK6 (14052-1-AP, Proteintech), CEP170 (18899-1-AP, Proteintech), PARP (9542 S, Cell Signaling Technology), cleaved caspase-3 (9661 S, Cell Signaling Technology), ac4C (ab252215, Abcam), rabbit IgG (7074, Cell Signaling Technology), β-actin (60008-1-Ig, Proteintech), Ki67 (AF0198, Affinity), α-tubulin (ab7291, Abcam), BrdU mAb (MI-11-3, MBL), secondary antibodies included goat anti-rabbit IgG(H+L) HRP (FMS-Rb01, Fcmacs) or mouse (S0002, Affinity). Remodelin was purchased from CSNpharm. Puromycin was from AEPxBIO (B7587), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, M8180) was purchased from Solarbio. Actinomycin D was purchased from MCE (MedChemExpress, HY-17559). 5′-Bromo-uridine (BrU) was purchased from Sigma–Aldrich (850187). Lipofectamine Transfection Reagent (40802ES02), TRIeasy Total RNA Extraction Reagent (19201ES60), complementary DNA synthesis superMix (11123ES10), and SYBR Green Master Mix (11198ES03) were purchased from Yeasen.
Complementary dna synthesis supermix
Manufactured by Yeasen
Sourced in China
Complementary DNA synthesis superMix is a premixed solution designed for the efficient synthesis of complementary DNA (cDNA) from RNA templates. The solution contains all the necessary components, including reverse transcriptase, RNase inhibitors, and optimized buffers, to generate high-quality cDNA in a single-step reaction.
Automatically generated - may contain errors
Lab products found in correlation
Trieasy total rna extraction reagent, by Yeasen (2 mentions)
Sybr green master mix, by Yeasen (2 mentions)
Ab252215, by Abcam (1 mentions)
Ab7291, by Abcam (1 mentions)
5 bromouridine, by Merck Group (1 mentions)
Mouse s0002, by Affinity Biosciences (1 mentions)
Remodelin, by CSNpharm (1 mentions)
Lipofectamine transfection reagent, by Yeasen (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
β actin 60008 1 ig, by Proteintech (1 mentions)
Puromycin, by Beyotime (1 mentions)
Transfection reagent, by Yeasen (1 mentions)
Doxycycline, by Beyotime (1 mentions)
Anti vegf, by Proteintech (1 mentions)
Senescence associated β galactosidase sa β gal kit, by Beyotime (1 mentions)
Annexin 5 fitc, by BioLegend (1 mentions)
Dutasteride, by Apexbio (1 mentions)
Goat anti rabbit igg h l hrp, by Fcmacs (1 mentions)
Anti nuclear factor κb nf κb p65, by Cell Signaling Technology (1 mentions)
Rnase a, by Yeasen (1 mentions)
2 protocols using complementary dna synthesis supermix
1
Antibody-Based Protein Analysis Protocol
2
Antibody-based Cellular Pathway Analysis
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The following antibodies were used: anti-SRD5A1 (Proteintech, #66329-1-Ig, 1:1,000 dilution), anti-nuclear factor-κB (NF-κB) p65 (Cell Signaling Technology, #8242, 1:1,000 dilution), anti-Phospho-NF-κB p65 (Cell Signaling Technology, #3033, 1:1,000 dilution), anti-VEGF (Proteintech, #19003-1-AP, 1:1,000 dilution), and anti-β-actin (Proteintech, #66009-1-Ig, 1:5,000 dilution). Secondary antibodies included goat anti-Rabbit IgG(H+L) HRP (Fcmacs, #FMS-Rb01, 1:5,000 dilution) or mouse (Affinity, #S0002, 1:10,000 dilution). 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Solarbio (Beijing, China), dutasteride was purchased from APExBIO (Houston, TX, USA), propidium iodide (PI) was purchased from XunBei (Nanjing, China), and Annexin-V-FITC was purchased from Biolegend (San Diego, CA, USA). Pierce TM BCA Protein Assay kit, transfection reagent, and RNase A were purchased from YEASEN (Shanghai, China). Senescence-associated β-galactosidase (SA-β-gal) kit, puromycin, and doxycycline were purchased from Beyotime Institute of Biotechnology (Shanghai, China). TRIeasy™ Total RNA Extraction Reagent, complementary DNA synthesis superMix, and SYBR Green Master Mix were purchased from YEASEN (Shanghai, China).
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