CD24-negative mature thymocytes were enriched by magnetic depletion of CD24+ cells, as previously described [14 (link)]. In brief, total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20 × 106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 × 106 cells) for 30 mins on ice. After washing off excess reagents, thymocytes were mixed with anti-rat IgG-conjugated BioMag beads (QIAgen) and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec). Anti-CD24 antibody-bound cells were then magnetically removed, and non-binding cells were harvested for further experiments.
Rat anti mouse cd24 antibodies m1 69
Manufactured by BioLegend
Rat anti-mouse CD24 antibodies (M1/69) are primary antibodies that recognize the CD24 antigen expressed on the surface of various mouse cell types, including hematopoietic cells. These antibodies can be used in flow cytometry, immunohistochemistry, and other immunological applications to detect and characterize CD24-positive cells.
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2 protocols using rat anti mouse cd24 antibodies m1 69
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Enrichment of CD24-negative Thymocytes
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Enrichment of CD24-negative Thymocytes
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CD24-negative mature thymocytes were enriched by magnetic depletion of CD24 + cells, as previously described (Park,Kwon, et al., 2019) . In brief, total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×10 6 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 µg/100 ×10 6 cells) for 30 mins on ice. After washing off excess reagents, thymocytes were mixed with anti-rat IgG-conjugated BioMag beads (QIAgen) and incubated for 45 mins at 4℃ on a MACSmix Tube Rotator (Miltenyi Biotec).
Anti-CD24 antibody-bound cells were then magnetically removed, and non-binding cells were harvested for further experiments.
Anti-CD24 antibody-bound cells were then magnetically removed, and non-binding cells were harvested for further experiments.
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