Optical rotations were measured in CH2Cl2 on a PerkinElmer 241 polarimeter (Waltham, MA, USA) using an Na lamp. NMR spectra were recorded on a Bruker AVANCE 500 MHz (Bruker Biospin, Fällanden, Switzerland). NMR spectra were obtained dissolving samples in CDCl3 (99.9%) and chemical shifts are reported relative to solvent (δH 7.26 and δC 77.0 ppm) and TMS as an internal pattern. HR-ESI-MS data were obtained on an LCT Premier XE Micromass spectrometer (Waters, Milford, CT, USA). Thin-layer chromatography (TLC; Merck, Darmstadt, Germany) was visualized by spraying with cobalt chloride reagent 2% (10% sulfuric acid) and heating.
Paclitaxel, cisplatin, DMEM/F12 medium, fetal bovine serum, gentamicin, penicillin–streptomycin, and Alamar Blue™ were obtained from Invitrogen (Gran Island, NY, USA). The reagents for general use were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Paclitaxel, cisplatin, DMEM/F12 medium, fetal bovine serum, gentamicin, penicillin–streptomycin, and Alamar Blue™ were obtained from Invitrogen (Gran Island, NY, USA). The reagents for general use were purchased from Sigma-Aldrich (St. Louis, MO, USA).