The materials used were the steams of T. asiatica (collected from Okinawa Island Japan), and Merck silica gel 60 (particle size 0.063-0.200 mm, 70-230 mesh. 250 mm). Analytical TLC was performed using Kieselgel 60 F254 DC-fertigplatten (Merck). Microbial strains used were E. coli, S. aureus, A. niger, Cladosporium sp., and C. cucurbitarum (supplied by Natural Product Laboratory, Faculty of Science, University of the Ryukyus).
Kieselgel 60 f254 dc fertigplatten
Manufactured by Merck Group
Kieselgel 60 F254 DC-fertigplatten is a pre-coated thin-layer chromatography (TLC) plate used for analytical and preparative applications. It consists of a silica gel layer with fluorescent indicator F254 coated on an aluminum sheet. The silica gel layer serves as the stationary phase for chromatographic separations.
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2 protocols using kieselgel 60 f254 dc fertigplatten
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Phytochemical Screening and Antimicrobial Evaluation
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Analytical Techniques for Chemical Characterization
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Optical rotation was measured using a JASCO P-1010 Polarimeter. Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker Avance III 500 spectrometer in CDCl 3 .
Chemical shifts and coupling constants were given as δ and Hz, respectively, and 1 H and 13 C chemical shifts were referenced to the residual solvent peaks (δ H = 7.26 and δ C = 77.24).
Infrared (IR) spectra were recorded on a JASCO FT/IR-6100 Fourier Transform Infrared Spectrometer. High-resolution mass spectra (HRMS) were obtained on an LTQ Orbitrap hybrid mass spectrometer (Thermo Scientific, Bremen, Germany) equipped with a nanospray ionizat ion (NSI) source . Open column chromatography was performed on Kieselgel 60 (70-230 mesh, Merck). Highperformance liquid chromatography (HPLC) was performed using a COSMOSIL Si60 HPLC column (5SL, 10 × 250 mm). Analytical thin-layer chromatography (TLC) was performed using Kieselgel 60 F 254 DC-fertigplatten (Merck). All solvents were reagent grade.
Chemical shifts and coupling constants were given as δ and Hz, respectively, and 1 H and 13 C chemical shifts were referenced to the residual solvent peaks (δ H = 7.26 and δ C = 77.24).
Infrared (IR) spectra were recorded on a JASCO FT/IR-6100 Fourier Transform Infrared Spectrometer. High-resolution mass spectra (HRMS) were obtained on an LTQ Orbitrap hybrid mass spectrometer (Thermo Scientific, Bremen, Germany) equipped with a nanospray ionizat ion (NSI) source . Open column chromatography was performed on Kieselgel 60 (70-230 mesh, Merck). Highperformance liquid chromatography (HPLC) was performed using a COSMOSIL Si60 HPLC column (5SL, 10 × 250 mm). Analytical thin-layer chromatography (TLC) was performed using Kieselgel 60 F 254 DC-fertigplatten (Merck). All solvents were reagent grade.
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