Human primary pulmonary microvascular endothelial cells (HPMEC, ScienCell, Carlsbad, CA) derived from fetal lung tissue (gestational age 18–19 weeks, 2 female lots and 1 male lot), were used according to manufacturer’s instructions (8 (link)). HPMEC are representative of capillaries. E. Coli Ultrapure 055:B5 LPS (100ng/mL) was purchased from Invivogen (San Diego, CA). Recombinant human ANGPT1 (rhANGPT1, 200ng/mL) and recombinant human ANGPT2 (rhANGPT2, 25ng/mL) were purchased from R&D Systems. For experiments with rhANGPT1 in vitro, cells were pre-treated for 1h prior to the addition of LPS or rhANGPT2.
E coli ultrapure 055 b5 lps
Manufactured by InvivoGen
E. Coli Ultrapure 055:B5 LPS is a laboratory reagent derived from Escherichia coli 055:B5 strain. It is a purified lipopolysaccharide (LPS) that can be used for research purposes.
Automatically generated - may contain errors
2 protocols using e coli ultrapure 055 b5 lps
1
Human Pulmonary Microvascular Endothelial Cell Stimulation
2
Human Pulmonary Microvascular Endothelial Cell Stimulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human primary pulmonary microvascular endothelial cells (HPMEC, ScienCell, Carlsbad, CA) derived from fetal lung tissue (gestational age 18–19 weeks, 2 female lots and 1 male lot), were used according to manufacturer’s instructions (8 (link)). HPMEC are representative of capillaries. E. Coli Ultrapure 055:B5 LPS (100ng/mL) was purchased from Invivogen (San Diego, CA). Recombinant human ANGPT1 (rhANGPT1, 200ng/mL) and recombinant human ANGPT2 (rhANGPT2, 25ng/mL) were purchased from R&D Systems. For experiments with rhANGPT1 in vitro, cells were pre-treated for 1h prior to the addition of LPS or rhANGPT2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!