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6 protocols using 1 myristoyl 2 palmitoyl sn glycero 3 phosphocholine

1

Lipid-based Contrast Agent Preparation

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Dipalmitoyl phosphatidylcholine (DPPC), 1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000] (DSPE-PEG2000) were bought from Avanti Polar Lipids (Alabaster, AL). Mag (gadopentate dimeglumine) was bought from Bayer HealthCare Pharmaceuticals Inc. (Wayne, NJ). All solvents used were of analytical grade.
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2

Lipid-based Nanoparticle Formulation

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Dipalmitoyl phosphatidylcholine (DPPC),1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000 (DSPE—PEG2000) and 1,2-dipalmitoyl-sn-glycero-3-phospoethanolamine-N-diethylenetriaminepentaacetic acid (gadolinium salt) Gd-DSPE were purchased from Avanti Polar Lipids (Alabaster, AL). Gadopentate dimeglumine (Magnevist®) was purchased from Bayer HealthCare Pharmaceuticals Inc. (Wayne, NJ). MiaPaCa-2 cell line was bought from American Type Culture Collection (ATCC) (Manassas, VA). All solvents used were of analytical grade.
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3

Liposomal Gemcitabine Delivery for Pancreatic Cancer

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Dipalmitoylphosphatidilcholine (DPPC),1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000 (DSPE PEG2000), 1,2-distearoyl-sn-glycero-3-phosphacholine (DSPC), Cholesterol (CHOL) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissaminerhodamine B sulfonyl) (ammonium salt) (DOPERho) were purchased from Avanti Polar Lipids (Alabaster, AL). Gemcitabine hydrochloride (2′-Deoxy-2′,2′-difluorocytidine) was bought from Sigma-Aldrich (St. Louis, MO). MiaPaCa-2, BxPC-3, AsPC and PANC-1 cell lines were purchased from American Type Culture Collection (ATCC) (Manassas, VA). All solvents used were of analytical grade.
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4

Lipid-based Contrast Agent Preparation

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Dipalmitoyl phosphatidylcholine (DPPC), 1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000] (DSPE-PEG2000) were bought from Avanti Polar Lipids (Alabaster, AL). Mag (gadopentate dimeglumine) was bought from Bayer HealthCare Pharmaceuticals Inc. (Wayne, NJ). All solvents used were of analytical grade.
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5

Liposomal Gemcitabine Delivery for Pancreatic Cancer

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Dipalmitoylphosphatidilcholine (DPPC),1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine (MPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol) 2000 (DSPE PEG2000), 1,2-distearoyl-sn-glycero-3-phosphacholine (DSPC), Cholesterol (CHOL) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissaminerhodamine B sulfonyl) (ammonium salt) (DOPERho) were purchased from Avanti Polar Lipids (Alabaster, AL). Gemcitabine hydrochloride (2′-Deoxy-2′,2′-difluorocytidine) was bought from Sigma-Aldrich (St. Louis, MO). MiaPaCa-2, BxPC-3, AsPC and PANC-1 cell lines were purchased from American Type Culture Collection (ATCC) (Manassas, VA). All solvents used were of analytical grade.
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6

Lipid Vesicle Preparation and Biotin-Streptavidin Conjugation

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PBS tablets were purchased
from Merck. Milli-Q grade water was used where applicable. 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1-myristoyl- 2-palmitoyl-sn-glycero-3-phosphocholine (MPPC), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl)
(DOPE-biotin) were purchased from Avanti Polar Lipids, Inc. Streptavidin
(SAv) from Streptomyces avidinii was
purchased from Merck.
All materials were used as received.
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