TPA, CAT, and cis-cis MA samples were collected after culture centrifugation at 15,000 × g for 10 min. Then, supernatants were diluted 50 times before being injected in the HPLC (Shimadzu, Japan) equipped with a UV detector. The related metabolites were separated on a C18 LC column (Phenomenex Luna 5 µm C18 (2), 100 Å, 150 × 4.6 mm, Torrance, CA) at 40 °C and a flow rate of 0.5 mL/min. The following gradients of buffer A (0.005% Formic acid) and buffer B (20% Acetonitrile) were used: buffer B was first increased from 60% to 80% for 2 min, then ramped to 100% for 6 min, and held at 100% for 12 min. TPA, CAT, and MA were detected at 260 nm with the quantifications being realized using external standards. Shimadzu LCsolution was used to collect the HPLC data. The standard curves were generated by measuring 0.001–0.1 g/L disodium terephthalate (99.0 + %, TCI America™), 0.05–0.5 g/L CAT (≥99.0%, Sigma Aldrich), and 0.001–0.1 g/L MA ((≥97.0%, Sigma Aldrich), respectively.
Disodium terephthalate
Manufactured by Tokyo Chemical Industry
Sourced in United States
Disodium terephthalate is a chemical compound with the formula Na2C8H4O4. It is a crystalline solid that is used as a raw material in the production of various polymers and resins.
Automatically generated - may contain errors
Lab products found in correlation
Luna 5 m c18 2, by Phenomenex (1 mentions)
Lc solution, by Shimadzu (1 mentions)
High performance liquid chromatography (hplc), by Shimadzu (1 mentions)
200 proof ethanol, by Decon Labs (1 mentions)
Nitric acid, by Thermo Fisher Scientific (1 mentions)
Hi 3220, by Hanna Instruments (1 mentions)
2 2 2 trifluoroethanol, by Merck Group (1 mentions)
2 protocols using disodium terephthalate
1
HPLC Quantification of TPA, CAT, and cis-cis MA
2
Synthesis and Characterization of Disodium Terephthalate
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Disodium terephthalate, and 2-hydroxyterephthalic acid were purchased from TCI America (Portland, OR, USA). 2,2,2-Trifluoroethanol, ammonium sulfate crystals, and 0.1 N sulfuric were purchased from Sigma-Aldrich. Nitric acid (70% trace metal grade) was purchased from Fisher Scientific (Pittsburg, PA, USA). Ethanol (200 proof) was purchased from Decon Labs.
A rigorous cleaning process including acid soaking and multiple rinses was followed for all glass and Teflon containers, as detailed in Kuang et al. (2017) (link). 18.2 M cm -1 MilliQ water was used for cleaning and to prepare reagent solutions. pH was measured with a bench top pH meter (HANNA instruments, HI 3220), calibrated daily with pH 4, 7, and 10 standards.
A rigorous cleaning process including acid soaking and multiple rinses was followed for all glass and Teflon containers, as detailed in Kuang et al. (2017) (link). 18.2 M cm -1 MilliQ water was used for cleaning and to prepare reagent solutions. pH was measured with a bench top pH meter (HANNA instruments, HI 3220), calibrated daily with pH 4, 7, and 10 standards.
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