96 block module

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The 96-block module is a laboratory equipment designed to hold 96 samples or reagents in a standardized microplate format. It provides a consistent and organized platform for various analytical and experimental procedures within a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy mini kit, by Qiagen (4 mentions) Abi prism 7500, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

96-well block module 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module Fast 96-Well Block Module

4 protocols using 96 block module

Quantitative Gene Expression Analysis using qPCR

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RNA was isolated using RNeasy Mini kit (Qiagen) and mRNA quantitation was performed using SYBR Green in an ABI PRISM 7500 sequence detection system with 96-block module and automation accessory (Applied Bio-system). GAPDH or ß-actin was used as an internal control. All samples were analyzed in triplicate. The primer sequences are listed in the Table 2.Table 2

The primer sequences used for gene expression analysis using qPCR

SETD1A	Forward	5′-GGCCAGATTCATCAACCACT-3′
SETD1A	Reverse	5′-CGATCTTCTTCTGGGACTCG-3′
SKP2	Forward	5′-ATGCCCCAATCTTGTCCATCT-3′
SKP2	Reverse	5′-CACCGACTGAGTGATAGGTGT-3′
GAPDH	Forward	5′-AGTCCTTCCACGATACCAAAGT-3′
GAPDH	Reverse	5′-CATGAGAAGTATGACAACAGCCT-3′
β-Actin	Forward	5′-CTCTTCCAGCCTTCCTTCCT-3′
β-Actin	Reverse	5′-AGCACTGTGTTGGCGTACAG-3′
BTG2	Forward	5′-CAGAGCACTACAAACACCACTG-3′
BTG2	Reverse	5′-CTGAGTCCGATCTGGCTGG-3′

Tajima K., Matsuda S., Yae T., Drapkin B.J., Morris R., Boukhali M., Niederhoffer K., Comaills V., Dubash T., Nieman L., Guo H., Magnus N.K., Dyson N., Shioda T., Haas W., Haber D.A, & Maheswaran S. (2019). SETD1A protects from senescence through regulation of the mitotic gene expression program. Nature Communications, 10, 2854.

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RNA Isolation and Gene Expression Analysis

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RNA was isolated using RNeasy Mini kit (Qiagen) and mRNA quantitation was performed using SYBR Green in an ABI PRISM 7500 sequence detection system with 96-block module and automation accessory (Applied Bio-system). GAPDH was used as an internal control. All samples were analyzed in triplicate. The primer sequences are listed in the Table S3.

Antonishyn N.A., McDonald R.R., Chan E.L., Horsman G., Woodmansee C.E., Falk P.S, & Mayhall C.G. (2000). Evaluation of Fluorescence-Based Amplified Fragment Length Polymorphism Analysis for Molecular Typing in Hospital Epidemiology: Comparison with Pulsed-Field Gel Electrophoresis for Typing Strains of Vancomycin-Resistant Enterococcus faecium. Journal of Clinical Microbiology, 38(11), 4058-4065.

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Quantitative Real-Time RT-PCR Analysis

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RNA was isolated using RNeasy Mini kit (Qiagen, Hilden, Germany) and used for real-time quantitative reverse transcription (qRT)-PCR using SYBR Green in an ABI PRISM 7500 sequence detection system with a 96-block module and automation accessory (Applied Biosystem, Karlsruhe, Germany). Glyceraldehyde 3-phosphate dehydrogenase was used as an internal control gene. All samples were analyzed in triplicate. The primer sequences are listed in Supplementary Table 1.

Li X., Gao D., Wang H., Li X., Yang J., Yan X., Liu Z, & Ma Z. (2015). Negative feedback loop between p66Shc and ZEB1 regulates fibrotic EMT response in lung cancer cells. Cell Death & Disease, 6(4), e1708-.

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RNA Isolation and Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated using RNeasy Mini kit (Qiagen) and mRNA quantitation was performed using SYBR Green in an ABI PRISM 7500 sequence detection system with 96-block module and automation accessory (Applied Bio-system). GAPDH was used as an internal control. All samples were analyzed in triplicate. The primer sequences are listed in the Table S3.

Matsuda S., Revandkar A., Dubash T.D., Ravi A., Wittner B.S., Lin M., Morris R., Burr R., Guo H., Seeger K., Szabolcs A., Che D., Nieman L., Getz G.A., Ting D.T., Lawrence M.S., Gainor J., Haber D.A, & Maheswaran S. (2023). TGF-β in the microenvironment induces a physiologically occurring immune-suppressive senescent state. Cell reports, 42(3), 112129.

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