Emeprid

Cats of both studies received approximately 100 T. brevior L3 on SD 0 as described below. Animals were anesthetized with a combined intramuscular injection of 0.08 ml/kg body weight (BW) Domitor^® (1 mg/ml medetomidine HCl, Zoetis) and 0.075 ml/kg BW Ketamin 10%^® (100 mg/ml ketamine HCl, WDT). After deep anesthesia, the cat received 0.06 ml/kg BW Emeprid^® IM (5 mg/ml, metoclopramide HCl, CEVA) 15 min (Study 1) or a few minutes (Study 2) before inoculation to prevent vomiting or regurgitation. A stomach tube was inserted without (Study 1) or with a rigid endoscope (Study 2). The inoculum was applied via syringe directly into the stomach, the tube was flushed with tap water and pulled out after confirming that no inoculation suspension remained in the tube. All cats were observed for vomiting or regurgitation directly after inoculation for up to 1 h (± 10 min) post-infection.

Anti-emetic or placebo administration was completed at t_1h following cisplatin treatment. Depending on treatment allocation from the experimental design, dogs received one of the following: 0.5 mg/kg ondansetron (Zofran®, GSK, Brentford, UK), 1 mg/kg maropitant (Cerenia®, Zoetis, Florham Park, NJ, USA), 0.5 mg/kg metoclopramide (Emeprid®, Ceva Santé Animale, Amersham, UK) or 0.9% saline as placebo. All treatments were made up to a standard volume of 15 mL using 0.9% saline and were administered intravenously through the second lumen of the jugular catheter at a flow rate of 1.0 mL/min.