For HBV, women were tested using the Murex HBsAg Version 3 with Confirmatory Assay (Murex Biotech, Dartford, UK). The presence of HBeAg was assessed using the ARCHITECT HBeAg Reagent Kit (Abbott Diagnostics Division, Wiesbaden, Germany). Serum HBV-DNA was quantified using the COBAS TaqMan HBV Test (Roche Molecular System, Branchburg, NJ, USA) with a limit of quantification of 6 IU/ml. For HCV all sera were first tested with the Innotest HCV Ab IV assay (Innogenetics, Ghent, Belgium). Low and high-positive results were re-assayed using the Ortho HCV 3.0 ELISA test (Ortho-Clinical Diagnostics, Raritan, NJ, USA). Samples reactive with both assays were considered anti-HCV positive [11 (link)]. In anti-HCV positive women the presence of HCV-RNA was tested in at least 3 different samples using the Versant HCV-RNA 1.0 assay (Siemens Diagnostics) with a detection limit of 15 IU/ml. Only patients with detectable HCV-RNA were considered HCV-positive.
Architect hbeag reagent kit
Manufactured by Abbott
Sourced in Germany
The ARCHITECT HBeAg Reagent Kit is a laboratory diagnostic product developed by Abbott. It is designed to detect the presence of hepatitis B e antigen (HBeAg) in human serum or plasma samples. The kit provides the necessary reagents and materials to perform this specific immunoassay test.
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2 protocols using architect hbeag reagent kit
1
Viral Hepatitis Diagnostic Protocols
2
Quantification of HBV DNA, RNA, and Antigens
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total DNA was extracted with a QIAamp DNA Blood Mini Kit (Qiagen) from 100 μL of the culture supernatant or 1.0 × 107 cells, and 5 μL of the extracted DNA solution was subjected to RT‐PCR using a LightCycler 480 system (Roche Diagnostics) with SYBR Green I Master (Roche Diagnostics), primers HBXF11 (5′‐ATG GCT GCT AGG CTG TGC TG‐3′) and HBXR4 (5′‐GTC CGC GTA AAG AGA GGT GC‐3′). Total RNA was extracted from 1.0 × 107 cells using Trizol (Invitrogen) and subjected to cDNA synthesis by SuperScript III (Invitrogen). RT‐PCR for quantifying total amount of pregenomic RNA and precore messenger RNA (mRNA) was conducted with SYBR Green I Master, primers HBCF7 (5′‐GCT ACC TGG GTG GGT GGT AAT TTG G‐3′) and HBCR5 (5′‐AGG GGA CCT GCC TCG TCG TCT AAC‐3′). The HBsAg and hepatitis B e antigen (HBeAg) levels in the culture supernatant were quantified with a chemiluminescence immunoassay (CLIA) using the ARCHITECT HBsAg reagent kit and ARCHITECT HBeAg reagent kit (Abbott Laboratories, Chicago, IL), respectively.
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